Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb #9624

general   motif   PBS   PKA   rabbit monoclonal   RRXS   RRXT   substrate  

No. Size Price
9624L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9624S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9624 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG
IP 1:50
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb detects peptides and proteins containing a phospho-Ser/Thr residue with arginine at the -3 and -2 positions. It is a useful tool in identifying new substrates of PKA. The antibody recognizes other -3 arginine-bearing phospho-Ser/Thr peptides, such as substrate motifs for Akt and PKC, to a lesser extent. It does not recognize the nonphosphorylated substrate motif peptides. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb兔单抗识别包含精氨酸-3和-2位上有磷酸化丝氨酸/苏氨酸的肽段或蛋白质。它是识别PKA底物的有用工具。该抗体也可以以稍弱的强度识别包含-3位上有精氨酸的磷酸化丝氨酸/苏氨酸的肽段,如Akt和PKC的底物模体。它不能识别非磷酸化的底物模体蛋白。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及其对应的国外部分)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-PKA substrate peptides .

该单克隆抗体用合成的磷酸化PKA底物肽段免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, phosphorylated in vitro with PKA kinase or treated in culture with forskolin/IBMX, using Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb. Lysis buffer: 1.0% Triton X-100 (lanes 1 and 2), 2.0% SDS (lanes 3 and 4).

对血清饥饿处理的A431细胞抽提液,使用PKA在体外磷酸化,或forskolin/IBMX培养,使用Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb进行Western blot分析。裂解缓冲液:1.0% Triton X-100 (列1和列2), 2.0% SDS (列3和列4)。

ELISA-Peptide

ELISA-Peptide

Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)

Phospho-PKA Substrate (RRXS*/T*) (100G7E) Rabbit mAb ELISA分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

Background

An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。磷酸化PKA底物特异性抗体由Cell Signaling Technology公司研发,用于研究PKA磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications