Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) PKA Substrate Antibody #9621

general   motif   PBS   phospho serine   phospho-serine   phosphoserine   PKA   RXXS   RXXT   substrate  

No. Size Price
9621L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9621S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9621 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,All Species Expected, Endogenous Rabbit
IP 1:100
IHC-P 1:200
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) PKA Substrate Antibody detects peptides and proteins containing a phospho-serine/threonine residue with arginine at the -3 position. It is a useful tool in identifying substrates of AGC family kinases, including PKA and PKC. It does not cross-react with the nonphosphorylated PKA substrate motif. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) PKA Substrate Antibody识别包含-3位上有精氨酸的磷酸化丝氨酸/苏氨酸残基的肽段或蛋白。它是识别AGC家族激酶(包括PKA和PKC)底物的有用工具。它不能识别非磷酸化的PKA底物基序。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phospho-PKA substrate peptide. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体用人工合成磷酸化PKA底物肽段免疫动物而制备。该抗体使用蛋白A和蛋白亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) PKA Substrate Antibody.

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) PKA Substrate Antibody进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, phosphorylated in vitro by protein kinase A, Erk2 or cdc2/cyclin A, plus or minus PKA inhibitor (PKI), using Phospho-(Ser/Thr) PKA Substrate Antibody.

对体外蛋白激酶A、Erk2或cdc2/cyclin A磷酸化,加或不加PKA抑制剂(PKI)的A431细胞,使用Phospho-(Ser/Thr) PKA Substrate Antibody进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser/Thr) PKA Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)

Phospho-(Ser/Thr) PKA Substrate Antibody ELISA分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phsopho-(Ser/Thr) PKA Substrate Antibody.

对石蜡包埋的人乳腺癌对照(左)和λ磷酸酶处理(右),使用Phsopho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.

对石蜡包埋的人肺癌使用Phospho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) PKA Substrate Antibody.

对石蜡包埋的人结肠癌使用Phospho-(Ser/Thr) PKA Substrate Antibody进行免疫组化分析。

Background

An important class of kinases, refered to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Phospho-PKA substrate-specific antibodies from Cell Signaling Technology are powerful tools for investigating the regulation of phosphorylation by PKA and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。磷酸化PKA底物特异性抗体由Cell Signaling Technology公司研发,用于研究PKA磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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