Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser) Kinase Substrate Antibody Sampler Kit #9615

kit   motif   phospho-ser   phospho-serine   substrate  

REACTIVITY

No. Size Price
9615T 1 Kit ( 4 x 20 µl ) ¥3,876.00 现货查询 购买询价
Kit Includes Quantity Applications Reactivity Homology† MW (kDa) Isotype
Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 20 µl W,IP,IHC-P,E-P, H,All, Rabbit IgG
Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981 20 µl W,IP,E-P, H,M,All, Rabbit
Anti-rabbit IgG, HRP-linked Antibody #7074 100 µl W, Goat
Anti-mouse IgG, HRP-linked Antibody #7076 100 µl W, Horse
Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix #9477 20 µl W,IP,E-P, H,All, Rabbit IgG
Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 20 µl W,IP,E-P, H,All, Mouse IgG1

Specificity / Sensitivity

Each antibody detects endogenous levels of phospho-(Ser) proteins of specific kinase substrate groups. For more detailed information please see datasheet for this product.

各抗体识别内源性特异激酶底物中含磷酸化丝氨酸的蛋白。更多信息请参考此产品的数据页。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with phosphopeptides containing the kinase substrate motif and purified by protein A and peptide affinity chromatography.Monoclonal antibody is produced by immunizing animals with synthetic phospho-MAPK/CDK substrate peptides.Mouse monoclonal antibody is produced by immunizing animals with phospho-(Ser) 14-3-3 binding motif peptides.

多克隆抗体使用包含激酶底物基序的磷酸化肽段免疫动物制备,通过蛋白A和肽段亲和色谱纯化。单克隆抗体使用合成的磷酸化MAPK/CDK底物肽段免疫动物制备。鼠单抗(IgM)使用包含磷酸化丝氨酸的14-3-3结合基序的肽段免疫动物制备。

Description

The Phospho-(Ser) Kinase Substrate Antibody Sampler Kit provides a fast and economical means of evaluating several phospho-Ser kinase substrates. The kit contains enough primary and secondary antibody to perform four Western blot experiments.

Phospho-(Ser) Kinase Substrate Antibody Sampler Kit提供了一种快速经济评估磷酸化丝氨酸激酶底物的方法。该试剂盒包含足够四次Western blot的一抗和二抗。

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

一抗结合靶蛋白后,加入二抗,HRP偶联的二抗复合物形成。然后加入LumiGLO*显色发光

Western Blotting

Western Blotting

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

一抗结合靶蛋白后,加入二抗,HRP偶联的二抗复合物形成。然后加入LumiGLO*显色发光

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) or Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right).

对A431细胞,未处理或calyculin A处理,抽提物使用Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) 或Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right)进行免疫印迹分析

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A treated, using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody.

对A431细胞,未处理或calyculin A处理,抽提物使用Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody进行免疫印迹分析

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA分析:磷酸化和非磷酸化的信噪比。(S*代表磷酸化的丝氨酸)

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

对血清饥饿处理的A431细胞,未处理或EGF培养,以及Hela细胞,未处理或羟基脲处理(G1/S)或诺考达唑处理(G2/M),抽提物使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

免疫组化分析石蜡包埋的人结肠癌,使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

免疫组化分析石蜡包埋的人肺癌,使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, control (left) or lambda phosphatase-treated (right), using Phospho-MAPK/CDK Substrates (PXS*P or S*PXR/K) (34B2) Rabbit mAb.

免疫组化分析石蜡包埋的人乳腺癌,使用的抗体为hospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb

Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved A431 cells, untreated or treated in culture with EGF, and HeLa cells, untreated, hydroxyurea-treated (G1/S) or Nocodazole-treated (G2/M), using Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325.

对血清饥饿处理的A431细胞,未处理或EGF培养,以及Hela细胞,未处理或羟基脲处理(G1/S)或诺考达唑处理(G2/M),抽提物使用Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-MAPK/CDK Substrate (PXS*P or S*PXR/K) (34B2) Rabbit mAb #2325 ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A treated using Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981.

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody #2981进行Western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 (left) or Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right).

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606(左)或Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601(右)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606 ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb.

对COS-7细胞,将其同步在G1/S、G2/M和G0期,使用Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477 ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477.

对COS-7细胞,将其同步在G1/S、G2/M和G0期,使用Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb #9477进行Western blot分析。

Background

Phospho-(Ser) kinases and phosphatases play critical roles in a wide range of biological processes. Each phospho-(Ser) kinase phosphorylates serine within a specific motif. The MAPK and CDK families of serine protein kinases phosphorylate serine followed by proline residue (1-3). The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids and S* is the phosphorylation site (4-6). MAPK phosphorylates substrates with the concensus sequence PX(S*)P. The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (7-11). Binding of 14-3-3 is mediated through phospho-serine-containing proteins (12). Two different phospho-serine containing motifs are found using a degenerate phospho-serine-oriented peptide library technique, RSXS*XP and RXY/FXS*XP (12). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FoxO3A, PKC and c-Raf (11, 13). Motif 2 (RXY/FXS*XP) is found in critical regulatory proteins including cdc25A, cdc25B, PKCγ, IRS-1 and BCR (12). Although Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody binds 14-3-3 binding motif 2 with no requirement for proline in the +2 position, it provides a powerful tool for the discovery and characterization of potential 14-3-3 binding motif 2-containing proteins or other proteins with the RXY/FXS* motif. Antibodies specific to particular kinase substrates are invaluable reagents in determining kinase activity and identifying potential new kinase substrates. CST has developed antibodies that recognize phosphorylated serine within the context of a protein motif that is phosphorylated by MAPK/CDK, CDKs or 14-3-3. As shown by DELFIA or ELISA, each phospho-(Ser) kinase substrate antibody in this sampler kit is specific to its kinase substrate motif.

磷酸化丝氨酸激酶和磷酸酶在生物过程中扮演重要角色。每个磷酸化丝氨酸激酶磷酸化特异性基序中的丝氨酸。MAPK和CDK丝氨酸蛋白激酶家族磷酸化脯氨酸残基之前的丝氨酸(1-3)。CDK底物的共同氨基酸序列为(K/R)(S*)PX(K/R),X代表任意20种氨基酸之一,S*是磷酸化位点(4-6)。MAPK磷酸化含有一致序列PX(S*)P的底物。14-3-3蛋白是参与调解细胞生存、凋亡、增殖和检查点控制的一组高度保守的蛋白家族(7-11)。14-3-3的结合由包含磷酸化丝氨酸的蛋白质介导(12)。两种包含磷酸化丝氨酸的基序在简并磷酸化导向肽库技术中发现,分别RSXS*XP和RXY/FXS*XP(12)。基序1(-3和-2位上精氨酸/赖氨酸和丝氨酸,0位磷酸化丝氨酸,+2位脯氨酸)在很多重要的调节蛋白包括Bad、cdc25C、Fox03A、PKC和cRaf中发现(11,13)。基序2(RXY/FXS*XP)在很多重要的调节蛋白包括cdc25A、cdc25B、PKCγ、IRS-1和BCR中发现(12)。尽管Phospho-(Ser) Arg-X-Tyr/Phe-X-pSer Motif Antibody可以结合14-3-3结合基序2,并且不需要+2位上的脯氨酸,它仍然是发现和鉴定潜在包含14-3-3结合基序2蛋白或其它含RXY/FXS*基序蛋白的有力工具。某些激酶底物有特异性的抗体对于研究激酶活性和发现潜在底物具有很高价值。CST开发了能够识别包含MAPK/CDK、CDK或14-3-3可以磷酸化的基序中磷酸化丝氨酸和苏氨酸的这些抗体。通过DELFIA或ELISA证实,此试剂盒中每个磷酸化丝氨酸激酶底物抗体都有特异性的目标激酶底物基序。

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications