Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614

AKT   akt1   akt2   akt3   general   motif   PBS   rabbit monoclonal   RXRXXS   RXRXXT   substrate  

No. Size Price
9614L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9614S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9614 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,D. melanogaster,All Species Expected, Endogenous Rabbit IgG
IP 1:50
IHC-P 1:1000
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*)(110B7) RmAb recognizes peptides and proteins containing phospho-serine/threonine preceded by arginine at the -3 position. There is some preference observed for peptides that contain phospho-serine/threonine preceded by arginine at both positions -5 and -3. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) Akt Substrate Motif (RXXS*/T*) (110B7) Rabbit mAb兔单抗识别包含-3位上是精氨酸的磷酸化丝氨酸/苏氨酸的肽段和蛋白。此抗体与包含-5、-3位上是精氨酸的磷酸化丝氨酸/苏氨酸肽段有偏好的交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-Akt substrate peptides .


Western Blotting

Western Blotting

Western blot analysis of extracts from serum-starved NIH/3T3 cells, phosphorylated in vitro with Akt kinase, or treated in culture with PDGF or FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

对血清饥饿处理的NIH/3T3细胞抽提液,使用Akt在体外磷酸化,或PDGF或FBS/Calyculin A培养,使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行Western blot分析。



Immunoprecipitation of extracts from serum-starved NIH/3T3 cells, untreated or treated with FBS/Calyculin A, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb, followed by Western blot analysis using the same antibody.

对血清饥饿的NIH/3T3细胞抽提液,未处理或使用FBS/Calyculin A处理,使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫共沉淀,然后用相同的抗体进行Western blot分析后。



Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)

Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb ELISA分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded lung carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

对石蜡包埋的人肺癌使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded renal cell carcinoma, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

对石蜡包埋的人肾癌使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb.

对石蜡包埋的人乳腺癌对照(左)和λ磷酸酶处理(右),使用Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb进行免疫组化分析。


An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。Akt在介导细胞内关键反应中扮演重要角色,包括细胞生长和生存、血管生成和转录调节(3-5)。目前已经发现一些Akt底物(如GSK-3、Bad、caspase-9),但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸(6)。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发,用于研究Akt磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
  4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
  5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
  6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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