Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser/Thr) Akt Substrate Antibody #9611

AKT   AKT1   akt2   akt3   general   motif   PBS   phospho serine   phospho-serine   phosphoserine   PKB   RAC   RAC-PK   RAC-PK-alpha   RXRXXS   RXRXXT   substrate  

No. Size Price
9611L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9611S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9611 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,All Species Expected, Endogenous Rabbit
IP 1:50
IHC-P 1:500
E-P 1:500

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser/Thr) Akt Substrate Antibody preferentially recognizes peptides and proteins containing phospho-Ser/Thr preceded by Lys/Arg at positions -5 and -3, in a manner largely independent of other surrounding amino acids. Some cross-reactivity is observed for peptides that contain phospho-Ser/Thr preceded by Arg/Lys at positions -3 and -2. No cross-reactivity is observed with the corresponding nonphosphorylated sequences or with other phospho-Ser/Thr-containing motifs. By ELISA, the antibody recognizes a wide range of phosphorylated Akt substrate peptides, and, by 2-D gel Western blot analysis, it recognizes a large number of proteins presumed to be Akt substrates. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser/Thr) Akt Substrate Antibody优先识别包含-5、-3位置上有赖氨酸/精氨酸的磷酸化丝氨酸/苏氨酸的内源性蛋白质,与周围序列无关。此抗体与-3位、-2位上是精氨酸/赖氨酸的磷酸化丝氨酸/苏氨酸的蛋白质有较弱的交叉反应。没有发现其能与相应的非磷酸化的序列或其它基序中的磷酸化丝氨酸/苏氨酸序列发生交叉反应。在ELISA中,其可以识别多种磷酸化Akt底物的肽段,在二维凝胶Western Blot中,其可以识别很多预测的Akt底物。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with phospho-Akt substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes prior to lysis), using Phospho-(Ser/Thr) Akt Substrate Antibody. Proteins were separated by 2-D electrophoresis prior to blotting.

对未处理或0.1uM calyculin A裂解前处理30分钟的Jurkat细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。蛋白质在印记前在二维电泳分离。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser/Thr) Akt Substrate Antibody.

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。



Phospho-(Ser/Thr) Akt Substrate Antibody ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* and S* denote phosphorylated threonine and serine.)

Phospho-(Ser/Thr) Akt Substrate Antibody ELISA分析:磷酸化和非磷酸化的信噪比。(T*和S*代表磷酸化的苏氨酸和丝氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Phospho-(Ser/Thr) Akt Substrate Antibody.

对石蜡包埋的人肺癌使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-(Ser/Thr) Akt Substrate Antibody.

对石蜡包埋的人结肠癌使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, untreated or insulin-treated (100 nM for 15 minutes prior to lysis), using Phospho-(Ser/Thr) Akt Substrate Antibody.

对未处理或100 nM 胰岛素裂解前处理15分钟的Jurkat细胞抽提液使用Phospho-(Ser/Thr) Akt Substrate Antibody进行Western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-(Ser/Thr) Akt Substrate Antibody.

对石蜡包埋的人乳腺癌对照(左)和λ磷酸酶处理(右),使用Phospho-(Ser/Thr) Akt Substrate Antibody进行免疫组化分析。


An important class of kinases, referred to as Arg-directed kinases or AGC-family kinases, includes cAMP-dependent protein kinase (PKA), cGMP-dependent protein kinase (PKG), protein kinase C, Akt, and RSK. These kinases share a substrate specificity characterized by Arg at position -3 relative to the phosphorylated Ser or Thr (1,2). Akt plays a central role in mediating critical cellular responses including cell growth and survival, angiogenesis, and transcriptional regulation (3-5). While a number of Akt substrates are known (such as GSK-3, Bad, and caspase-9) many important substrates await discovery. Akt phosphorylates substrates only at Ser/Thr in a conserved motif characterized by Arg at positions -5 and -3 (6). Phospho-Akt substrate-specific antibodies from CST are powerful tools for investigating the regulation of phosphorylation by Akt and other Arg-directed kinases, as well as for high throughput kinase drug discovery.

精氨酸导向的激酶或AGC家族激酶是很重要的激酶,包括cAMP依赖的蛋白激酶(PKA)、cGMP依赖的蛋白激酶(PKG)、蛋白激酶C、Akt和RSK。这些激酶有相同的底物特异性,-3位上有精氨酸的丝氨酸和苏氨酸(1,2)。Akt在介导细胞内关键反应中扮演重要角色,包括细胞生长和生存、血管生成和转录调节(3-5)。目前已经发现一些Akt底物(如GSK-3、Bad、caspase-9),但还有很多重要底物等待发现。Akt磷酸化保守序列-5、-3位是精氨酸的丝氨酸/苏氨酸(6)。磷酸化Akt底物特异性抗体由Cell Signaling Technology公司研发,用于研究Akt磷酸化调节和精氨酸导向的激酶,也可以用于高通量药物发现。

  1. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
  2. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  3. Marte, B.M. and Downward, J. (1997) Trends Biochem Sci 22, 355-8.
  4. Jiang, B.H. et al. (2000) Proc Natl Acad Sci USA 97, 1749-53.
  5. Scheid, M.P. and Woodgett, J.R. (2000) Curr Biol 10, R191-4.
  6. Alessi, D.R. et al. (1996) FEBS Lett 399, 333-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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