Cell Signaling Technology

Product Pathways - Screening Technologies

Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) MultiMab™ Rabbit mAb mix #9607

KinomeView   Motif antibody  

No. Size Price
9607S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9607 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 All Species Expected, Endogenous Rabbit IgG
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-ATM/ATR Substrate Motif (S*Q) (D23H2/D69H5) Rabbit mAb recognizes peptides and proteins containing sequences of phospho-Ser followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser containing motifs.

Phospho-ATM/ATR Substrate Motif (S*Q) (D23H2/D69H5) Rabbit mAb磷酸化ATM/ATR底物基序兔单抗识别包含+1位谷氨酰胺磷酸化丝氨酸的肽段和蛋白。此抗体不与相应的非磷酸化序列或其它包含磷酸化丝氨酸的基序发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the S*Q motif sequence. The antibody is formulated from two rabbit monoclones in order to cover a broad range of reactivity.

该单克隆抗体用合成的含S*Q基序序列的肽段免疫动物制备。该抗体是两个兔单克隆的混合,以便可以覆盖更大的反应范围。

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (+, 2 hr), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).

对未处理(-)或UV(两小时,+)处理30分钟的HeLa细胞抽提液使用Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnologies)。

IP

IP

Immunoprecipitation of HeLa cells, untreated (-) or UV-treated (+, 2 hr) (lanes 3 and 4), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody (upper) and Chk1 (2G1D5) Mouse mAb #2360 (lower).

对未处理(-)或UV(两小时,+)处理(列3和列4)的HeLa细胞抽提液使用Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb进行免疫共沉淀分析。10%输入显示在列1和2。Western Blot使用相同抗体(上)和Chk1 (2G1D5) Mouse mAb #2360(下)。

Background

Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.

共济失调毛细血管扩张症突变蛋白激酶(ATM)和共济失调毛细血管扩张症Rad3相关激酶(ATR)是与细胞周期检查点和DNA修复相关的激酶(1)。已发现的ATM底物包括p53、p95/NBS1、MDM2、Chk2、BRCA1、CtIP、4E-BP1和Chk1(1,2)。ATM/ATR底物的核心要求是S*/T*Q。-3和-1位的疏水氨基酸和+1位的负电荷氨基酸对这些激酶底物识别有正向决定作用。S*/T*Q附近的正电荷残基对底物磷酸化有负向决定作用(3)。AT细胞的复杂表型提示它们还有更多底物(3)。为了更好的理解激酶和鉴别ATM和ATR的相关底物,CST开发了识别S*/T*Q基序中磷酸化丝氨酸和苏氨酸的抗体。

  1. Kastan, M.B. and Lim, D.S. (2000) Nature Rev. Mol. Cell Biol. 1, 179-186.
  2. Zhao, H. and Piwnica-Worms, H. (2001) Mol. Cell. Biol. 21, 4129-4139.
  3. Kim, S. T. et al. (1999) J. Biol. Chem. 274, 37538-37543.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

LI-COR is a registered trademark of LI-COR, Inc.

Odyssey is a registered trademark of LI-COR, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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