Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb #9606

14-3-3 binding motif   general   KXXSXP   motif   PBS   phospho serine   phospho-serine   phosphoserine   RXXSXP   substrate  

No. Size Price
9606S 100 µl ( 40 western blots ) ¥3,900.00 现货查询 购买询价
9606 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:4000 Human,All Species Expected, Endogenous Mouse IgG1
IP 1:20
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb binds peptides and proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. By ELISA, the antibody recognizes a wide range of peptides containing this phosphorylated 14-3-3 binding motif in a manner that is phospho-specific and largely independent of the surrounding amino acid sequence. The antibody weakly cross-reacts with sequences containing phospho-Thr instead of phospho-Ser in this motif, and with sequences containing phospho-Ser surrounded by Phe at the +1 position and Arg/Lys at the -3 position. No cross-reactivity is observed with corresponding nonphosphorylated sequences or with other phospho-Ser/Thr/Tyr containing motifs. Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb complements our polyclonal Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 by showing slightly different and overlapping specificity. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb(鼠单抗)结合包含+2位脯氨酸和-3位精氨酸或赖氨酸的磷酸化丝氨酸肽段或蛋白。在ELISA中,该抗体可以识别多种包含磷酸化14-3-3结合基序的肽段,具有磷酸化特异性、并在很大程度上与周围氨基酸序列无关。该抗体与基序中包含磷酸化苏氨酸而非磷酸化丝氨酸的序列有弱相互作用,并与包含+1位有苯丙氨酸或-3位有精氨酸/赖氨酸的磷酸化丝氨酸肽段有弱相互作用。没有发现其能与相应的非磷酸化的序列或其它含磷酸化丝氨酸/苏氨酸/赖氨酸的基序发生交叉反应。Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb(鼠单抗)通过少量的变化和重叠的特异性补充了我们多克隆抗体Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及其对应的国外相应专利)。

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-(Ser) 14-3-3 binding motif peptides .

该单克隆抗体用磷酸化丝氨酸14-3-3结合基序多肽免疫动物制备。

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or calyculin A-treated, using Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb (left) or Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601 (right).

对未处理或calyculin A处理的A431细胞抽提液使用Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb(左)或Phospho-(Ser) 14-3-3 Binding Motif Antibody #9601(右)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-14-3-3 binding motif peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) 14-3-3 Binding Motif (4E2) Mouse mAb ELISA分析:磷酸化和非磷酸化14-3-3结合模体的信噪比。(S* 代表磷酸化的丝氨酸)

Background

The 14-3-3 proteins are a highly conserved family of proteins involved in the regulation of cell survival, apoptosis, proliferation and checkpoint control (1-5). Biological regulation by 14-3-3 is mediated through phosphorylation-dependent protein-protein interactions (6). Two different phospho-Ser-containing motifs are found within nearly all known 14-3-3 binding proteins (7). Motif 1 (Arg/Lys and Ser at positions -3 and -2, phospho-Ser at position 0, and Pro at position +2) is found in critical regulatory proteins including Bad, cdc25C, FKHRL1, PKC and c-Raf (5,7). Phospho-(Ser) 14-3-3 Binding Motif Polyclonal and (4E2) Monoclonal Antibodies provide powerful tools for the discovery and characterization of potential 14-3-3 binding proteins containing this motif and for high throughput drug discovery.

14-3-3蛋白是参与调控细胞生存、凋亡、增殖和检查点控制的一组高度保守的蛋白家族(1-5)。14-3-3的生物调控由磷酸化依赖的蛋白-蛋白相互作用介导(6)。两种不同的包含磷酸化丝氨酸的基序在几乎所有的14-3-3结合蛋白中发现(7)。基序1(精氨酸/赖氨酸和丝氨酸在-3和-2位,磷酸化丝氨酸在0位,脯氨酸在+2位)在很多重要的调节蛋白包括Bad、cdc25C、FKHRL1、PKC和cRaf中发现(5-7)。Phospho-(Ser) 14-3-3 Binding Motif Polyclonal and (4E2) Monoclonal Antibody(结合基序多克隆和单克隆抗体)提供了发现和鉴定潜在的含此基序的14-3-3结合蛋白、及高通量药物发现的有力工具。

  1. Aitken, A. (1995) Trends Biochem Sci 20, 95-7.
  2. Zha, J. et al. (1996) Cell 87, 619-28.
  3. Piwnica-Worms, H. (1999) Nature 401, 535, 537.
  4. Tzivion, G. et al. (1998) Nature 394, 88-92.
  5. Xing, H. et al. (2000) EMBO J 19, 349-58.
  6. Muslin, A.J. et al. (1996) Cell 84, 889-97.
  7. Yaffe, M.B. et al. (1997) Cell 91, 961-71.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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