Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) #9602

active caspase 3   active caspase-3   anti caspase 3   anti caspase-3   Apopain   caspase 3   caspase-3   caspase3   cleaved caspase 3   cleaved caspase-3   CPP 32   CPP-32   CPP32   SCA-1   Yama  

No. Size Price
9602S 100 µl ( 50 tests ) ¥3,986.00 现货查询 购买询价
9602 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
F 1:50 Human, Endogenous Rabbit IgG
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine, Pig,

Specificity / Sensitivity

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody detects nonspecific caspase substrates by western blot.

Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) 兔单抗仅能够检测内源性水平的Asp175磷酸化的Caspase-3蛋白。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.

该单克隆抗体是采用合成的人源caspase-3蛋白Asp175周围残基相对应的肽段免疫动物而制备的。

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.

该CST抗体标记了Alexa Fluor® 647荧光染料,通过了内部测试可以直接用于人细胞的免疫荧光试验。该抗体与不带标记的Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗 #9579有着相同的种属交叉反应。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine #9953 (1 μM, 4 hr; right), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 Phalloidin #8878 (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.

共聚焦免疫荧光分析HeLa细胞,未处理(左)和 Staurosporine #9953(1 μM, 4 hr;右)处理,使用抗体为Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb 兔单抗(Alexa Fluor® 647 Conjugate)(蓝色伪彩)。Alexa Fluor® 488 Phalloidin #8878(绿色)标记肌动蛋白丝。红色= Propidium Iodide (PI)/RNase Staining Solution #4087.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with Etoposide #2200 (green), using Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate).

流式细胞术检测Jurkat细胞,未处理(蓝)和 etoposide #2200 处理(绿),使用抗体为Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Alexa Fluor® 647 Conjugate)兔单抗。

Background

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Caspase-3 (CPP-32, Apoptain, Yama, SCA-1)是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚(ADP-ribose)聚合酶(PARP)(1)。caspase-3的活化需要经过其未激活的的酶原转变成活化的 p17 和 p12 片段的蛋白水解过程。caspase-3的剪切需要天门冬氨酸位于p1位点(2)。

  1. Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
  2. Nicholson, D.W. et al. (1995) Nature 376, 37-43.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols


For Research Use Only. Not For Use In Diagnostic Procedures.

The Alexa Fluor dye antibody conjugates in this product are sold under license from Life Technologies Corporation for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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