Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MSK1 (Thr581) Antibody #9595

mitogen stress kinase   msk1  

No. Size Price
9595S 100 µl ( 10 western blots ) ¥4,050.00 现货查询 购买询价 防伪查询
9595T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价 防伪查询
9595 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 90 Rabbit
IP 1:200
IHC-P 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-MSK1 (Thr581) Antibody detects endogenous levels of MSK1 only when phosphorylated at Thr581. This antibody does not cross-react with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3.

Phospho-MSK1 (Thr581) Antibody兔多抗能检测内源性Thr581位点磷酸化的MSK1蛋白水平。该抗体不能与其他位点磷酸化的MSK1蛋白发生交叉反应,也不能检测磷酸化的RSK1, RSK2和RSK3蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr581 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, transfected with wt MSK1 or mutant MSK1, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物:转染MSK1、突变体MSK1的293细胞和未处理、TPA处理的(200 nM)HeLa细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using Phospho-MSK1 (Thr581) Antibody.免疫组织化学方法检测石蜡包埋的人乳腺癌组织,显示了细胞核位点,使用的抗体为Phospho-MSK1 (Thr581) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, untreated, UV-treated or calyculin A-treated, using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物:未处理、紫外照射和calyculin A处理的293细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells untreated or treated with UV and HeLa cells untreated or treated with calyculin A, using Phospho-MSK1 (Thr581) Antibody.Western blot方法检测细胞提取物:未处理、紫外照射293细胞和未经处理、calyculin A处理的HeLa细胞。使用的抗体是Phospho-MSK1 (Thr581) Antibody。


MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

MSK1是一种有丝分裂原和应激活化的蛋白激酶,能够被Erk、p38 MAPK活化,分别应答生长因子和细胞应激反应的刺激(1)。MSK1与RSK类似,它有两个激酶区域,这两个区域由调节链接区域连接(2)。RSK1蛋白中Ser364和Ser381位点的磷酸化对RSK1的活性非常重要。MSK1的Ser360和Ser376位点与RSK1中的这两个位点同源,可能对MSK1的活性也同样重要。

  1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
  2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
  3. Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496-1505.
  4. Markou, T. and Lazou, A. (2002) Biochem J 365, 757-63.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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