Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MSK1 (Ser360) Antibody #9594

mitogen stress kinase   MSK1  

No. Size Price
9594S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9594 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 90 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-MSK1 (Ser360) Antibody detects transfected MSK1 only when phosphorylated at Ser360. This antibody does not recognize MSK1 phosphorylated at other sites, nor does it cross-react with phosphorylated RSK1, RSK2 or RSK3.

Phospho-MSK1 (Ser360) Antibody兔多抗能检测Ser360位点磷酸化的MSK1蛋白。该抗体不能识别其他位点磷酸化的MSK1蛋白,也不能与磷酸化的RSK1, RSK2和RSK3发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser360 of human MSK1. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells transfected with MSK1 or various mutants of MSK1, then treated with TPA (200 nM) and analyzed using Phospho-MSK1 (Ser360) Antibody.Western blot方法检测细胞提取物:转染MSK1和各种MSK1突变体的TPA (200 nM)处理的293细胞。使用的抗体是Phospho-MSK1 (Ser360) Antibody。

Western Blotting

Western Blotting

Western blot analysis of extracts from RSK1, RSK2, RSK3 or MSK1 transfected 293 cells, untreated or TPA-treated (200 nM), using Phospho-MSK1 (Ser360) Antibody.Western blot方法检测细胞提取物:转染RSK1、 RSK2、 RSK3 、MSK1,未处理和TPA (200 nM)处理的293细胞。使用的抗体是Phospho-MSK1 (Ser360) Antibody。


MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

MSK1是一种有丝分裂原和应激活化的蛋白激酶,能够被Erk、p38 MAPK活化,分别应答生长因子和细胞应激反应的刺激(1)。MSK1与RSK类似,它有两个激酶区域,这两个区域由调节链接区域连接(2)。RSK1蛋白中Ser364和Ser381位点的磷酸化对RSK1的活性非常重要。MSK1的Ser360和Ser376位点与RSK1中的这两个位点同源,可能对MSK1的活性也同样重要。

  1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
  2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
  3. Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496-1505.
  4. Markou, T. and Lazou, A. (2002) Biochem J 365, 757-63.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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