Cell Signaling Technology

Product Pathways - Development

β-Catenin Antibody (Carboxy-terminal Antigen) #9587

catenin   CTNNb   sc-7199  

No. Size Price
9587S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9587T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
9587 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 92 Rabbit
IP 1:50
IHC-P 1:1600
IHC-F 1:400
ChIP 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IHC-F=Immunohistochemistry (Frozen), ChIP=Chromatin IP,

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Dog, Pig, Horse,

Specificity / Sensitivity

Beta-Catenin Antibody detects endogenous levels of total β-catenin protein.

Beta-Catenin抗体识别内源性的总β-catenin蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminus of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体由合成肽段免疫动物产生,合成的肽段与人源β-catenin的羧基末端氨基酸残基序列一致,抗体由蛋白质A和肽段亲和层析纯化得到。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen) in the presence of control peptide (left) or beta-Catenin blocking peptide #1002 (right).

在对照多肽(左)或beta-Catenin封闭多肽#1002存在的条件下,使用 β-Catenin抗体(羧基末端抗原)对石蜡包埋的人乳腺癌组织进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of total cell extracts from 293 and NIH/3T3 cells using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对293和NIH/3T3细胞提取物进行western blot分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin Antibody (Carboxy-terminal Antigen).

beta-Catenin对石蜡包埋的人结肠癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对石蜡包埋的人肺癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma, using β-Catenin Antibody (Carboxy-terminal Antigen).

使用beta-Catenin对石蜡包埋的人透明细胞癌组织进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Apc (Min/+) mouse intestinal adenoma using β-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对石蜡包埋的Apc(Min/+)小鼠肠腺瘤组织进行免疫组化分析。

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen HCC827 xenograft using beta-Catenin Antibody (Carboxy-terminal Antigen).

使用β-Catenin抗体(羧基末端抗原)对冰冻的HCC827爪蟾进行免疫组化分析。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT116 cells and either 20 μl of β-Catenin Antibody (Carboxy-terminal Antigen) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003将4 x 106 HCT116细胞与20μl的β-Catenin 抗体 (羧基末端抗原)或2μl的Normal Rabbit IgG #2729交联然后进行染色质免疫共沉淀实验。富集的DNA使用SimpleChIP® Human Axin2 Intron 1 Promoter 引物 #8973, SimpleChIP® Human CaMK2D Intron 3 引物 #5111, human c-Myc promoter 引物和SimpleChIP® Human α Satellite Repeat 引物 #4486以real-time PCR的方式定量。各样品沉淀得到的DNA量相对于input染色质总量进行相对定量,Input中染色质量设定为1。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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