Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Drosophila Dcp-1 (Asp216) Antibody #9578

apoptosis   Dcp-1   death caspase-1   Drosophila  

No. Size Price
9578S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9578 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 D. melanogaster, Endogenous 22 Rabbit
IF-IC 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Drosophila Dcp-1 (Asp216) Antibody recognizes endogenous levels of the large 22 kDa fragment of cleaved Dcp-1. This antibody does not recognize full length Dcp-1. The antibody also detects a non-specific, apoptotic-related band at 50 kDa by western blot.

Cleaved Drosophila Dcp-1 (Asp216) Antibody能够检测内源性水平的Dcp-1剪切的22 kDa大片段。该抗体不能识别全长Dcp-1。通过western blot分析,该抗体也能检测一个非特异性凋亡相关的50 kDa 的条带。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp216 of Drosophila Dcp-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的果蝇Dcp-1蛋白近Asp216氨基末端残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Drosophila S2 cells, untreated (-) or treated with either cycloheximide (10 μM, 5 hr; +) or actinomycin D (0.7 μM, 5 hr; +), using Cleaved Drosophila Dcp-1 (Asp216) Antibody.

Western blot 检测果蝇S2细胞提取物,未处理 (-)或cycloheximide(放线菌酮) (10 μM, 5 hr; +) 或actinomycinD(放线菌素D)(0.7 μM, 5 hr; +)处理,使用抗体为Cleaved Drosophila Dcp-1 (Asp216) Antibody。



Confocal immunofluorescent analysis of wild-type (white1118) Drosophila egg chambers, conditioned (left) or conditioned followed by starvation (right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

免疫荧光共聚焦分析野生型(white1118)果蝇卵室,处理(左)或饥饿后处理(右),使用抗体为Cleaved Drosophila Dcp-1 (Asp216) Antibody(绿色)。DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。



Confocal immunofluorescent analysis of S2 cells, untreated (left) or treated with Actinomycin D (0.7 uM, 20 hr; right), using Cleaved Drosophila Dcp-1 (Asp216) Antibody (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析检测果蝇S2细胞提取物,未处理 (左)或Actinomycin D(放线菌素D)(0.7 μM, 20 hr; 右)处理,使用抗体为Cleaved Drosophila Dcp-1 (Asp216) Antibody。DY-554 phalloidin(红色)标记肌动蛋白丝。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。


Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases, and four encode effector caspases (reviewed in (2)). The Drosophila effector caspase, death caspase-1 (Dcp-1), is a critical executioner of apoptosis. It is involved in the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP). The activation of Dcp-1 requires proteolytic processing of its inactive zymogen into active p22 and p13 fragments (3). Comparison of the in vivo activity between DrICE and Dcp-1 has shown that DrICE is a more effective inducer of apoptosis than Dcp-1, which instead plays a role in determining the rate of cell death (4).

黑腹果蝇的细胞死亡和哺乳动物细胞死亡一样,是受许多相同的刺激调控的(1)。果蝇的基因组包含七个caspase基因,三个编码半胱天冬酶,四个编码效应胱天蛋白酶(2)。果蝇的效应器caspase,即死亡caspase-1(DCP-1),是一个关键的细胞凋亡执行者。它参与了许多关键蛋白的蛋白水解的剪切,如多聚核酶(ADP-核糖)聚合酶(PARP)。 DCP-1的激活需要其非活动状态酶原蛋白水解成活性p22和p13片段(3)。研究比较体内drICE和DCP-1的活性表明,drICE相对于Dcp-1是一种更有效的细胞凋亡诱导剂,后者在确定细胞死亡率方面起到了重要作用(4)。

  1. Steller, H. et al. (1994) Philos Trans R Soc Lond B Biol Sci 345, 247-50.
  2. Hay, B.A. and Guo, M. (2006) Annu Rev Cell Dev Biol 22, 623-50.
  3. Song, Z. et al. (1997) Science 275, 536-40.
  4. Florentin, A. and Arama, E. (2012) J Cell Biol 196, 513-27.

Application References

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