Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-eNOS (Thr495) Antibody #9574

endothelial nitric-oxide synthase  

No. Size Price
9574S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9574 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Bovine,Pig, Endogenous 140 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat,

Specificity / Sensitivity

Phospho-eNOS (Thr495) Antibody detects endogenous levels of eNOS only when phosphorylated at threonine 495.

Phospho-eNOS (Thr495) AntibodyAntibody可以检测Thr495位磷酸化的内源性总eNOS蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr495 of human eNOS. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人eNOS的Thr495周围序列相对应的磷酸肽免疫动物而生产的。抗体用蛋白A和肽亲和层析来纯化的。

Western Blotting

Western Blotting

Western blot analysis of extracts from porcine aortic endothelial cells (PAECs), 3 days post-confluence, untreated, H2O2-treated (0.3 mM for 30 minutes) or calf intestinal alkaline phosphatase (CIP)-treated as indicated, using Phospho-eNOS (Thr495) Antibody (upper) or control eNOS antibody (lower).

Western blot分析猪动脉内皮细胞(PAECs) ,汇合后3天,未处理组和H2O处理组(0.3 mM,30 分钟)或小牛肠碱性磷酸酶处理组,所用抗体为Phospho-eNOS (Thr495) Antibody (上)或对照 eNOS antibody (下)。

Background

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7).

内皮一氧化氮合酶(eNOS)是心血管系统的一种重要的酶。它可以催化合成一氧化氮(NO)。NO是一种血压,血管重建和生成的重要的调节因子(1,2)。eNOS的活性受多个位点磷酸化的调控。研究最为透彻的两个位点是激活位点Ser1177 和抑制位点Thr495(3)。几种蛋白激酶包括Akt/PKB, PKA 和AMPK都可以在不同的刺激下通过 Ser1177位点的磷酸化来激活eNOS的活性(4,5)。相反,血管舒缓激肽和过氧化氢通过促进Ser1177的磷酸化和Thr495的去磷酸化来激活eNOS的活性(6,7)。

  1. Fulton, D. et al. (2001) J Pharmacol Exp Ther 299, 818-24.
  2. Shaul, P.W. (2002) Annu Rev Physiol 64, 749-74.
  3. Chen, Z.P. et al. (1999) FEBS Lett 443, 285-9.
  4. Dimmeler, S. et al. (1999) Nature 399, 601-5.
  5. Fulton, D. et al. (1999) Nature 399, 597-601.
  6. Harris, M.B. et al. (2001) J Biol Chem 276, 16587-91.
  7. Thomas, S.R. et al. (2002) J Biol Chem 277, 6017-24.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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