Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser675) Antibody #9567

b-catenin   CTNNB   CTNNB1  

No. Size Price
9567S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9567 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Rat, Endogenous 92 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Chicken, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-β-Catenin (Ser675) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser675.

磷酸化 β-Catenin(Ser675)抗体只识别Ser675磷酸化的内源性β-Catenin。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin. Antibodies are purified by peptide affinity chromatography.

多克隆抗体由合成的磷酸化肽段免疫动物产生,合成的磷酸化肽段与人源β-catenin 邻近675位丝氨酸的氨基酸序列一致。抗体由蛋白质A和肽段亲和层析纯化得到。

Western Blotting

Western Blotting

COS-7 cells were transfected with cDNA encoding DYKDDDDK-tagged wild type (WT) β-catenin or Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting using Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine/Pulmonary, University of Chicago).

DYKDDDDK标记的野生型(WT)β-catenin或Ser-to-Ala β-catenin突变体cDNA转染COS-7细胞后如图所示。10 mM forskolin (FSK)处理细胞5分钟后,溶解的β-catenin 或其突变体用DYKDDDDK Tag抗体免疫沉淀(结合到same epitope as Sigma's Anti-FLAG® M2抗体)#2368并使用Phospho-β-Catenin (Ser552)抗体 #9566, Phospho-β-Catenin (Ser675) 抗体#9567, 或 DYKDDDDK Tag抗体进行western blot实验。(图片由芝加哥大学Department of Medicine / Pulmonary, Sebastien Taurin和 Nickolai Dulin博士提供)

Western Blotting

Western Blotting

Western blot analysis of extracts from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes or λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser675) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (lower).

使用hospho-β-Catenin (Ser675) 抗体(上)或 β-Catenin 抗体(氨基端抗原)#9581(下)对经过forskolin (FSK) 处理30分钟或λ磷酸酶处理1小时的SK-N-MC细胞进行western blot分析。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

PKA was shown to phosphorylate β-catenin at Ser675. Phosphorylation at Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9,10).

PKA能磷酸化β-catenin的675位丝氨酸。675位丝氨酸位点的磷酸化诱导β-连环蛋白在核内的累积,并增加其 转录活性[9,10].

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.
  10. Hino, S. et al. (2005) Mol. Cell Biol. 25, 9063-9072.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications