Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser552) Antibody #9566

catenin. beta   CTNNB   CTNNB1  

No. Size Price
9566S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9566 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse, Endogenous 92 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Rat, Chicken, Xenopus, Zebrafish,

Specificity / Sensitivity

Phospho-β-Catenin (Ser552) Antibody detects endogenous levels of β-catenin only when phosphorylated at Ser552.

Phospho-β-Catenin (Ser552) 抗体仅能识别552位丝氨酸被磷酸化后的内源性β-Catenin。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin. Antibodies are purified by peptide affinity chromatography.

合成与人β-catenin 552位丝氨酸及其邻近氨基酸残基序列一致的多肽,免疫动物获得多克隆抗体。抗体经肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from SK-N-MC cells, treated with forskolin (FSK) for 30 minutes, or the lysate was treated with λ phosphatase for 1 hour, using Phospho-β-Catenin (Ser552) Antibody (upper) or β-Catenin Antibody (Amino-terminal Antigen) #9581 (bottom).

使用Phospho-β-Catenin (Ser552) 抗体(上)或 β-Catenin 抗体 (氨基末端抗原) #9581 (底)对经过forskolin(FSK)处理30分钟或λ 磷酸酶处理1小时的SK-N-MC细胞提取物进行western blot分析。

Western Blotting

Western Blotting

COS-7 cells were transfected with cDNAs for the DYKDDDDK-tagged wild type (WT) β-catenin or for Ser-to-Ala β-catenin mutants as indicated. Cells were stimulated with 10 mM forskolin (FSK) for 5 minutes and lysed. β-catenin or its mutants were immunoprecipitated with DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 and analyzed by western blotting with Phospho-β-Catenin (Ser552) Antibody #9566, Phospho-β-Catenin (Ser675) Antibody #9567, or DYKDDDDK Tag Antibody as indicated (Figures provided by Drs. Sebastien Taurin and Nickolai Dulin, Department of Medicine / Pulmonary, The University of Chicago).

DYKDDDDK标记的野生型(WT)β-catenin或Ser-to-Ala β-catenin突变体cDNA转染COS-7细胞后如图所示。10 mM forskolin (FSK)处理细胞5分钟后,溶解的β-catenin 或其突变体用DYKDDDDK Tag抗体免疫沉淀(结合到same epitope as Sigma's Anti-FLAG® M2抗体)#2368并使用Phospho-β-Catenin (Ser552)抗体 #9566, Phospho-β-Catenin (Ser675) 抗体#9567, 或 DYKDDDDK Tag抗体进行western blot实验。(图片由芝加哥大学Department of Medicine / Pulmonary, Sebastien Taurin和 Nickolai Dulin博士提供)。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

Both Akt and PKA were shown to phosphorylate β-catenin at Ser552. Phosphorylation at Ser552 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-11).

Akt和PKA均能磷酸化β-catenin552位丝氨酸。552位丝氨酸磷酸化使β-catenin入细胞核并提高其转录活性(9-11)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.
  9. Taurin, S. et al. (2006) J. Biol. Chem. 281, 9971-9976.
  10. Fang, D. et al. (2007) J. Biol. Chem. 282, 11221-11229.
  11. He, X.C. et al. (2007) Nat. Genet. 39, 189-198.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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