Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Thr41/Ser45) Antibody #9565

beta catenin   CTNNB  

No. Size Price
9565S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9565T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9565 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Monkey, Endogenous 92 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Dog, Pig,

Specificity / Sensitivity

Phospho-β-Catenin (Thr41/Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at Thr41 or Ser45. This antibody does not recognize β-catenin phosphorylated at other sites.

Phospho-β-Catenin (Thr41/Ser45)抗体仅识别41位苏氨酸和45位丝氨酸磷酸化的内源性beta-catenin。本抗体并不识别其他位点磷酸化后的β-catenin.。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr41 and Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

合成对应人β-catenin 41位苏氨酸和45位丝氨酸的邻近氨基酸残基序列一致的磷酸肽段,免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Thr41/Ser45) Antibody (upper) or β-Catenin Antibody #9562 (lower).

使用Phospho-β-Catenin (Thr41/Ser45) 抗体 (上 )或 β-Catenin 抗体 #9562 (下),对20 mM LiCl处理30分钟和随后用50 nM calyculin A处理的293细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from SW480 cells using Phospho-β-Catenin (Thr41/Ser45) Antibody.

使用Phospho-β-Catenin (Thr41/Ser45)对SW480细胞提取物进行western blot分析。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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