Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser45) Antibody #9564

beta catenin  

No. Size Price
9564S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9564 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 92 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Dog, Pig,

Specificity / Sensitivity

Phospho-β-Catenin (Ser45) Antibody detects endogenous levels of beta-catenin only when phosphorylated at serine 45. It does not recognize β-catenin phosphorylated at other sites.

磷酸化 β- catenin (Ser45)抗体只识别Ser45磷酸化的内源性β- catenin。此抗体不识别其他位点磷酸化的β- catenin。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser45 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体由合成的磷酸化肽段免疫动物产生,合成的磷酸化肽段与人源β- catenin Ser45氨基酸残基序列一致。抗体由蛋白质A和肽段亲和层析纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells untreated and treated with 50 nM calyculin A for 30 minutes, using Phospho-β-Catenin (Ser45) Antibody (lanes 1-2) or the same antibody after preincubation with listed peptides (lanes 3-8). (S45-P is phospho-Ser45 peptide; S33/37/T41-P is phospho-Ser33/37/Thr41 peptide; S45-non-P is nonphospho-Ser45 peptide.)

使用Phospho-β-Catenin (Ser45) 抗体(1-2道)或与图示中的多肽提前孵育过的抗体(3-8道),对未处理或经过50 nM calyculin A处理30分钟的293细胞提取物进行western blot分析。(S45-P是 phospho-Ser45 peptide; S33/37/T41-P 是phospho-Ser33/37/Thr41 peptide; S45-non-P是nonphospho-Ser45 peptide.)

Western Blotting

Western Blotting

Western blot analysis of extracts from HEK293 cells untreated and treated with calyculin A (50 nM for 30 minutes), using Phospho-β-Catenin (upper) and β-Catenin Antibody #9562 (lower).

使用Phospho-β-Catenin (上)和 β-Catenin 抗体#9562 (下)对未处理和经过calyculin A (50 nM 30分钟)的HEK293细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from SW480 cells, using Phospho-β-Catenin (Ser45) Antibody.

使用Phospho-β-Catenin (Ser45)抗体对SW480细胞提取物进行western blot分析。

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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