Cell Signaling Technology

Product Pathways - Development

Phospho-β-Catenin (Ser33/37/Thr41) Antibody #9561

beta catenin  

No. Size Price
9561L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9561S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9561T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9561 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 92 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,


Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig,

Specificity / Sensitivity

Phospho-β-Catenin (Ser33/37/Thr41) Antibody detects endogenous levels of β-catenin only when phosphorylated at serines 33, 37 or threonine 41. It does not recognize beta-catenin phosphorylated at other sites.

磷酸化 β- catenin (Ser33/37/Thr41)抗体只检测发生在丝氨酸33,37或者苏氨酸41磷酸化的内源性β- catenin。此抗体不识别其他位点磷酸化的β- catenin。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体由合成的磷酸化肽段免疫动物产生,合成的磷酸化肽段与人源β- catenin 邻近33位,37位丝氨酸或41位苏氨酸的氨基酸残基序列一致。抗体由蛋白质A和肽段亲和层析纯化得到。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, pretreated with 20 mM LiCl for 30 minutes and then with 50 nM calyculin A, using Phospho-β-Catenin (Ser33/37/Thr41) Antibody (upper) or β-Catenin Antibody #9562 (lower).

使用Phospho-β-Catenin (Ser33/37/Thr41) 抗体 (上) 或 β-Catenin 抗体 #9562 (下)对经过 20 mM LiCl处理30分钟后和50 nM calyculin A处理的293细胞提取物进行western blot分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from SW480 cells using Phospho-β-Catenin (Ser33/37/Thr41) Antibody.

使用Phospho-β-Catenin (Ser33/37/Thr41)抗体对SW480细胞提取物进行western blot分析。


β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).

β-catenin是Wnt信号通路下游的重要效应分子(1)。在脊椎动物体内它涉及到两个重要的生物过程:早期胚胎发育(2)和肿瘤发生(3)。CK1可以磷酸化β-catenin 45位丝氨酸。该磷酸化将导致β-catenin能够随后被GSK-3磷酸化(4-6)。GSK-3β通过磷酸化β-catenin Ser33, Ser37, 和 Thr41,并促使其进一步被降解(7)。这些位点发生突变会提高β-catenin蛋白稳定性,并且已在多种癌细胞株中发现这些突变(8)。

  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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