Product Pathways - Cell Cycle / Checkpoint
RIF1 (D2F2M) Rabbit mAb #95558
|95558S||100 µl ( 10 western blots )||￥3,250.00 现货查询||购买询价|
|95558||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry),
Specificity / Sensitivity
RIF1 (D2F2M) Rabbit mAb recognizes endogenous levels of total RIF1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu2110 of human RIF1 protein.
Confocal immunofluorescent analysis of MCF7 (positive; left) and U-2 OS (lower expression; right) cells using RIF1 (D2F2M) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).
The Rap1 interacting factor 1 (RIF1) was originally identified as a regulator of telomere homeostasis in yeast and mammalian cells (1). Research studies show that RIF1 regulates the timing of eukaryotic DNA replication origin firing through its affect on chromatin architecture (2-4). Additional studies show that RIF1 is essential for regulating the repair of DNA double strand breaks (DSBs). RIF1 is recruited to sites of DSBs by 53BP1 in response to DNA damage, and suppresses 5' end resection to favor the non-homologous end joining (NHEJ) pathway over homologous recombination (HR) repair (5-9). Oct4 and Smad3 modulate Rif1 expression in mouse embryonic stem cells; Rif1 may regulate embryonic stem cell stability during cell proliferation (10).
- Miller, K.M. et al. (2005) EMBO J 24, 3128-35.
- Hayano, M. et al. (2012) Genes Dev 26, 137-50.
- Cornacchia, D. et al. (2012) EMBO J 31, 3678-90.
- Yamazaki, S. et al. (2012) EMBO J 31, 3667-77.
- Zimmermann, M. et al. (2013) Science 339, 700-4.
- Di Virgilio, M. et al. (2013) Science 339, 711-5.
- Chapman, J.R. et al. (2013) Mol Cell 49, 858-71.
- Escribano-Díaz, C. et al. (2013) Mol Cell 49, 872-83.
- Li, P. et al. (2015) Cell Death Dis 6, e1588.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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