Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) #9548

ADPRT   PARP apoptosis  

No. Size Price
9548S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9548T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9548 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 89 Mouse IgG2b

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) detects endogenous levels of the large fragment of mouse PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific) 鼠单抗能够检测内源的ASP214处裂解的小鼠PARP1大片段(89 kDa)。该抗体不能检测高含量的全长PARP或其它PARP亚型。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP.

该单克隆抗体是采用合成的鼠源PARP Asp214 周围C-端残基相应的肽免疫动物后制备获得。

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM), using Cleaved PARP (Asp214) (7C9) Mouse mAb (Mouse Specific).

Western blot检测 NIH/3T3 细胞,未处理或 staurosporine (星胞菌素 )(1 µM)处理,使用抗体Cleaved PARP (Asp214) (7C9) Mouse mAb 鼠单抗 (鼠种特异性)。

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)该产品通过了许可证(Promega Corp., U.S. Patent No. 6,350,452.)

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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