Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) #9546

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No. Size Price
9546L 600 µl ( 120 western blots ) ¥8,792.00 现货查询 购买询价
9546S 200 µl ( 40 western blots ) ¥3,900.00 现货查询 购买询价
9546T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9546 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human, Endogenous 89 Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 resulting from cleavage at aspartic acid 214. This antibody will detect high levels of full length PARP.

该单克隆抗体能够检测内源性的 ASP214 处裂解的人源 PARP1 大片段(89 kDa)蛋白水平。该抗体也能检测高含量的全长PARP。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic PARP peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP.

该单克隆抗体是用合成的人源PARP Asp214 C-端氨基序列相对应的肽段免疫动物后制备获得。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific).

采用Cleaved PARP (Asp214) (19F4) Mouse mAb (Human Specific)鼠单抗对未处理或依托泊苷(25 µM, 5hrs)处理的Jurkat细胞进行免疫印迹分析(western blot)。

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)该产品通过了许可证(Promega Corp., U.S. Patent No. 6,350,452.)

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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