Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) Antibody (Rat Specific) #9545


No. Size Price
9545S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9545T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9545 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 89 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved PARP (Asp214) Antibody (Rat Specific) detects endogenous levels of the large fragment of rat PARP1 (89 kDa) resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Cleaved PARP (Asp214) Antibody (Rat Specific)能够检测内源性的由caspase裂解PARP1生成的大片段。该抗体不识别PARP1全长蛋白或其它PARP亚型。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in rat PARP. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的大鼠PARP Asp214 C-末端序列相对应的肽段免疫动物而生产获得。抗体经过了蛋白A和肽亲和色谱法纯化。

Western Blotting

Western Blotting

Western blot analysis of C6 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Rat Specific).


PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)


  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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