Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) Antibody (Mouse Specific) #9544


No. Size Price
9544S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9544T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9544 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 89 Rabbit
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved PARP (Asp214) Antibody (Mouse Specific) detects endogenous levels of the large fragment (89 kDa) of mouse PARP1 resulting from caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoforms.

Cleaved PARP (Asp214) 抗体能够检测内源的caspase裂解的小鼠 PARP1(89 kDa)的大片段。该抗体与PARP1全长蛋白及其它PARP亚型不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in mouse PARP. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的小鼠 Asp214 C-末端残基序列相对应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和色谱纯化。

Western Blotting

Western Blotting

Western blot analysis of NIH/3T3 cells, untreated or staurosporine-treated (1 µM, 3hrs), using Cleaved PARP (Asp214) Antibody (Mouse Specific).

采用Cleaved PARP (Asp214) Antibody (Mouse Specific)对 NIH/3T3 细胞(未处理或 staurosporine (星胞菌素) (1 µM, 3hrs)处理)进行免疫印迹分析(western blot)



Confocal immunofluorescent analysis of 3T3-L1 cells, untreated (left) or staurosporine-treated (right), using Cleaved PARP (Asp214) Antibody (Mouse Specific) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

激光共聚焦免疫荧光图片显示3T3-L1 细胞,未处理(左)或 staurosporine (星胞菌素)处理(右)用,使用Cleaved PARP (Asp214) Antibody (鼠特异性)(绿色)。微丝用DY-554 phalloidin(鬼比环肽)(红色)标记。蓝色伪彩=DRAQ5®#4084(DNA荧光染料) 。


PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

该产品通过了许可证(Promega Corp., U.S. Patent No. 6,350,452.)。

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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