Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved PARP (Asp214) Antibody (Human Specific) #9541

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No. Size Price
9541L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9541S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9541T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9541 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 89 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved PARP (Asp214) Antibody (Human Specific) detects endogenous levels of the large fragment (89 kDa) of human PARP1 produced by caspase cleavage. The antibody does not recognize full length PARP1 or other PARP isoform.

Cleaved PARP (Asp214)抗体能够检测被内源性的caspase酶裂解的人 PARP1大片段,不能识别全长PARP1或其它PARP亚型。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues surrounding Asp214 in human PARP. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源PARP Asp214位末端氨基序列相对应的肽段免疫动物而生产制备,抗体通过蛋白A和肽亲和色谱法纯化。

Western Blotting

Western Blotting

Western blot analysis of Jurkat cells, untreated or etoposide-treated (25 µM, 5hrs), using Cleaved PARP (Asp214) Antibody (Human Specific).

采用抗体Cleaved PARP (Asp214) Antibody (Human Specific). 对未处理或 etoposide(依托泊苷)处理(25 µM, 5hrs)的Jurkat细胞进行Western Blot分析。

Background

PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).

PARP,是核多聚(ADP-核糖) 聚合酶,分子量116kDa,参与环境胁迫应答中的DNA修复(1)。在体外该蛋白可被许多ICE样 caspases裂解(2,3),在体内是caspase-3裂解的主要靶蛋白之一(4,5)。在人体中PARP在位点Asp214 和 Gly215被裂解,裂解后PARR的N末端DNA 结合结构域(24 kDa)与C端催化结构域(89 kDa) 分离(2,4)。PARP协助细胞维持生存能力;PARP的裂解促进细胞崩解并可以作为细胞凋亡的标记物(6)。

(This product is sold under license from Promega Corp., U.S. Patent No. 6,350,452.)

该抗体通过了Promega公司的许可,专利号6,350,452.

  1. Satoh, M.S. and Lindahl, T. (1992) Nature 356, 356-358.
  2. Lazebnik, Y. A. et al. (1994) Nature 371, 346-347.
  3. Cohen, G.M. (1997) Biochem. J. 326, 1-16.
  4. Nicholson, D. W. et al. (1995) Nature 376, 37-43.
  5. Tewari, M. et al. (1995) Cell 81, 801-809.
  6. Oliver, F.J. et al. (1998) J. Biol. Chem. 273, 33533-33539.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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