Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser198) Antibody #9529

cdc 25c   cdc25 c   cdc25-c   CDC25M1   Dual specificity phosphatase Cdc25C   M-phase inducer phosphatase 3  

No. Size Price
9529S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9529 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Transfected Only 75 Rabbit
F 1:25

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, F=Flow Cytometry,

Specificity / Sensitivity

Phospho-cdc25C (Ser198) Antibody detects levels of cdc25C only when phosphorylated at Ser198 and only when derived from a transfected DNA construct. Phospho-cdc25C (Ser198) Antibody能够识别外源性转染的丝氨酸(198位)磷酸化的cdc25C蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser198 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对cdc25C蛋白丝氨酸(198位)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Western Blotting

Western Blotting

Western blot analysis of GST-cdc25C, unphosphorylated or phosphorylated by HTScan® PLK1 Kinase #7611, using Phospho-cdc25C (Ser198) Antibody (upper) or GST (26H1) Mouse mAb #2624 (lower). western blot方法检测未磷酸化和用HTScan® PLK1 Kinase #7611磷酸化的GST-cdc25C,使用的抗体为Phospho-cdc25C (Ser198) Antibody (上图) 或 GST (26H1) Mouse mAb #2624 (l下图).

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells overexpressing full-length GST-cdc25C, untreated or treated with calyculin A #9902, using Phospho-cdc25C (Ser198) Antibody. western blot方法检测过表达全长GST-cdc25C的HeLa细胞提取物:未处理组和calyculin A #9902处理组,使用的抗体为Phospho-cdc25C (Ser198) Antibody。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated Jurkat cells, using Phospho-cdc25C (Ser198) Antibody versus propidium iodide (DNA content). The boxed population indicates phospho-cdc25C (Ser198)-positive cells. 流式细胞术分析未处理的Jurkat细胞,使用Phospho-cdc25C (Ser198) Antibody抗体,PI染色法检测细胞内DNA含量。框内的细胞群为phospho-cdc25C (Ser198)阳性细胞。

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活,该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化,而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时,cdc25C结合14-3-3蛋白家族的成员,扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。

During prophase, polo-like kinase 1 (PLK1) phosphorylates cdc25C at Ser198, causing translocation from the cytoplasm to the nucleus, where cdc25C can interact with cdc2/cyclin B to allow for progression through the remaining stages of mitosis (6). 在细胞分裂前期,polo-样激酶1(PLK1)磷酸化cdc25C蛋白丝氨酸198位,可使cdc25C从胞浆转位至胞核,磷酸化cdc25C在胞核内与cdc2/cyclin B相互作用,使有丝分裂的其他时相有序进行。

  1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
  6. Toyoshima-Morimoto, F. et al. (2002) EMBO Rep. 3, 341-348.

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