Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Ser216) Antibody #9528

9521   cdc25   M-phase inducer phosphatase 3  

No. Size Price
9528S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9528 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 60 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-cdc25C (Ser216) Antibody detects endogenous levels of cdc25C only when phosphorylated at Ser216. Phospho-cdc25C (Ser216) Antibody能够检测内源性丝氨酸(216位点)磷酸化的cdc25C蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser216 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对cdc25C蛋白丝氨酸(216位点)磷酸化肽段免疫动物,利用A蛋白和多肽亲和层析方法纯化生产的。

Western Blotting

Western Blotting

Western Blot analysis of HT29 cells, untreated, nocodazole-treated, and lambda phosphotase-treated, using Phospho-cdc25C (Ser216) antibody (upper), and cdc25C (5H9) Rabbit mAb, #4688, (lower). western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT29细胞提取物,使用的抗体为Phospho-cdc25C (Ser216) antibody (上图)和cdc25C (5H9) Rabbit mAb, #4688 (下图)

Western Blotting

Western Blotting

Western blot analysis of wild type GST-cdc25C and mutant GST-cdc25C Ser216Ala fusion proteins, untreated or phosphorylated by Chk2, using Phospho-cdc25C (Ser216) Antibody. Western blot方法检测未使用Chk2处理和用Chk2磷酸化的野生型GST-cdc25C及突变型GST-cdc25C Ser216Ala融合蛋白,使用的抗体为 Phospho-cdc25C (Ser216) Antibody。

Western Blotting

Western Blotting

Western blot analysis of GST-cdc25C phosphorylated by cdc2/cyclin B (New England Biolabs #P6020), Chk2 or both kinases, using Phospho-cdc25C (Ser216) Antibody (upper) or Phospho-cdc25C (Ser214) antibody (lower). Western blot方法检测cdc2/cyclin B (New England Biolabs #P6020)、Chk2或两者同时磷酸化的GST-cdc25C,使用的抗体为Phospho-cdc25C (Ser216) Antibody (上图) 或 Phospho-cdc25C (Ser214) antibody (下图).

Background

cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活,该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化,而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时,cdc25C结合14-3-3蛋白家族的成员,扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。

  1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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