Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-cdc25C (Thr48) Antibody #9527

cdc25   CDC25C  

No. Size Price
9527S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9527T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9527 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 75 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-cdc25C (Thr48) Antibody detects endogenous levels of cdc25C only when phosphorylated at Thr48. Phospho-cdc25C (Thr48) Antibody 能够检测内源性苏氨酸(48位)磷酸化的cdc25C蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr48 of human cdc25C. Antibodies are purified by protein A and peptide affinity chromatography. 该多克隆抗体是由合成的人源的针对苏氨酸(48位)磷酸化肽段免疫动物,采用A蛋白和多肽亲和层析技术生产的。

Western Blotting

Western Blotting

Western Blot analysis of HT29 cell extracts untreated, nocodazole-treated and lambda phosphotase-treated using Phospho-cdc25C (Thr48) (upper), and cdc25C (5H9) Rabbit mAb, #4688 (lower). western blot方法检测未处理、nocodazole处理和λ磷酸酶处理的HT29细胞提取物,使用的抗体为Phospho-cdc25C (Thr48) (上图),和cdc25C (5H9) Rabbit mAb, #4688 (下图)


cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5). cdc25蛋白磷酸酶负责cdc2的去磷酸化和激活,该过程是真核细胞进入有丝分裂的关键调节步骤(1)。整个分裂间期cdc25C丝氨酸216位发生c-TAK1引起的组成性磷酸化,而该位点的磷酸化在G2/M期检验点是不依赖DNA损伤的(2)。当216位丝氨酸磷酸化时,cdc25C结合14-3-3蛋白家族的成员,扣押胞质cdc25C从而阻止有丝分裂的过早发生(3)。检验点蛋白激酶Chk1和Chk2磷酸化cdc25C216位丝氨酸以响应DNA损伤(4,5)。

Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization on phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10). Cdc25C的完全激活需要12个以上不同位点的磷酸化,这些位点磷酸化依赖于cdc2/cyclin B 和 Polo-样 激酶, 以及肽酰-脯氨酰-异构酶(PPI) Pin1的作用(6,7)。Pin1包含一个结合phospho-Ser/Thr-Pro 位点的WW结构域和一个在phospho-Ser/Thr-Pro结合点诱导顺反式异构化的催化区域(8)。Cdc25C的苏氨酸48和67位点被磷酸化后可以直接与Pin1的WW结构域相互作用(9)。苏氨酸48位的磷酸化也调节cdc25C与CKS/p13SUC1的结合(10)。

  1. Jessus, C. and Ozon, R. (1995) Prog. Cell Cycle Res. 1, 215-228.
  2. Peng, C.Y. et al. (1997) Science 277, 1501-1505.
  3. Kumagai, A. and Dunphy, W.G. (1999) Genes Dev. 13, 1067-1072.
  4. Blasina, A. et al. (1999) Curr. Biol. 9, 1-10.
  5. Furnari, B. et al. (1999) Mol. Biol. Cell 10, 833-845.
  6. Izumi, T. and Maller, J.L. (1993) Mol. Biol. Cell 4, 1337-1350.
  7. Stukenberg, P. T. et al. (2001) Mol. Cell 7, 1071-1083.
  8. Yaffe, M. B. et al. (1997) Science 278, 1957-1960.
  9. Lu, P. J. et al. (1999) Science 283, 1325-1328.
  10. Landrieu, I. et al. (2001) J. Biol. Chem 276, 1434-1438.

Application References

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