Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) #9509

casp39   Casp9   Caspase 9  

No. Size Price
9509S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9509T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9509 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Mouse, Endogenous 37 Rabbit
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) detects endogenous levels of the 37kDa subunit of mouse caspase-9 only after after cleavage at aspartic acid 353. It does not cross-react with full length caspase-9 or with other caspases at endogenous levels.

该抗体能够特异性检测内源性在Asp353处裂解的鼠源caspase-9 大片段(37 kDa)。该抗体与全长的caspase-9或其他caspases不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp353 of mouse caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的鼠源caspase-9 Asp353位点周围氨基酸序列相对应的多肽免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from L929 cells, untreated or staurosporine-treated, using Cleaved Caspase-9 (Asp353) Antibody (upper) or Caspase-9 Antibody #9504 (lower).

Western blot分析未处理或 staurosporine (星胞菌素)处理的L929 细胞,抗体采用Caspase-9 Antibody (Asp353) (上) 或Caspase-9 Antibody #9504 (下).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3 cells, staurosporine-treated (left) or untreated (right), using Cleaved Caspase-9 (Asp353) Antibody (Mouse Specific) (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析staurosporine(星胞菌素)处理(左)或未处理(右)的NIH/3T3 细胞,抗体采用 Cleaved Caspase-9 (Asp353) Antibody (鼠种特异性) (green)(绿色)。微丝用Alexa Fluor® 555 phalloidin(鬼比环肽)(红色)标注。蓝色伪彩是DRAQ5® #4084 (DNA荧光染料)。

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后,细胞色素C从线粒体释放,这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化,包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处,产生p37亚单位,可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用,包括 caspase-3 and caspase-7,并启动凋亡级联反应,最终导致凋亡。

  1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
  2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
  3. Liu, X. et al. (1996) Cell 86, 147-157.
  4. Li, P. et al. (1997) Cell 91, 479-489.
  5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
  6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
  7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
  9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
  10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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