Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) #9507

Casp-9   casp39   caspase 9   ICE-LAP6   Mch-6  

No. Size Price
9507S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9507 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Rat, Endogenous 17, 38 Rabbit
IF-IC 1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Caspase-9 (Asp353) Antibody (Rat Specific) detects endogenous levels of the large fragment (17 kDa or 38 kDa with prodomain) of caspase-9 resulting from cleavage at aspartic acid 353. The antibody does not recognize full length caspase-9 or any other cleaved caspases.

该抗体能够检测内源的353位天冬氨酸处裂解的caspase-9(Asp353)大片段(17 kDa or 38 kDa with prodomain)。该抗体和全长 Caspase-9以及其他cleaved caspases不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp353 of rat caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的小鼠源caspase-9 Asp353位点氨基末端残基相对应的多肽免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。

Western Blotting

Western Blotting

Western blot analysis of C6 cells, untreated or cytochrome c-treated (1hr, 0.25 mg/ml), and PC12 cells, untreated or staurosporine-treated (3hrs, 1uM), using Cleaved Caspase-9 (Asp353) Antibody (Rat Specific).

Western blot检测 C6 cells,未处理或 cytochrome c (细胞色素C)(1hr, 0.25 mg/ml),PC12 细胞,未处理或 staurosporine(星胞菌素)(3hrs, 1uM)处理,使用抗体Cleaved Caspase-9 (Asp353) Antibody (大鼠特异性).

IF-IC

IF-IC

Confocal immunofluorescent analysis of C6 cells, untreated (left) or treated with Staurosporine #9953 (right), using Cleaved Caspase-9 (Asp353) (Rat Specific) Antibody (green). Actin filaments were labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光图片显示C6细胞,未处理(左)或 Staurosporine (星胞菌素)#9953 处理(右),用 Cleaved Caspase-9 (Asp353) (Rat Specific) Antibody(绿色)。微丝用DY-554555 phalloidin(鬼比环肽)标记(红色)。蓝色伪彩=DRAQ5® #4084(DNA荧光染料)。

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后,细胞色素C从线粒体释放,这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化,包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处,产生p37亚单位,可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用,包括 caspase-3 and caspase-7,并启动凋亡级联反应,最终导致凋亡。

  1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
  2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
  3. Liu, X. et al. (1996) Cell 86, 147-157.
  4. Li, P. et al. (1997) Cell 91, 479-489.
  5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
  6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
  7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
  9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
  10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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