Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-9 (Asp315) Antibody (Human Specific) #9505

Casp-9   casp39   caspase 9   ICE-LAP6   Mch-6  

No. Size Price
9505S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9505T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9505 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 35 Rabbit
IP 1:500

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Cleaved Caspase-9 (Asp315) Antibody (Human Specific) detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases.

该抗体能够检测内源性的人源caspase-9(Asp315)裂解大片段(37 kDa with prodomain/17 kDa)的,与全长 Caspase-9以及其他caspase裂解片段无交叉反应.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

该单克隆抗体是采用合成的人caspas-9 Asp315 周围残基相对应的多肽免疫的动物而制备。抗体通过蛋白A和肽段亲和层析而纯化.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Cleaved Caspase-9 (Asp315) Antibody (Human Specific) (upper) or Caspase-9 Antibody (Human Specific) #9502 (lower).

Western blot 分析未处理或cytochrome c(细胞色素C)(0.25 mg/ml)处理的 Jurkat 细胞提取物,以及未处理或staurosporine(星胞菌素) (1 µM)或cytochrome c(细胞色素C)(0.25 mg/ml)处理的HeLa 细胞,抗体采用Cleaved Caspase-9 (Asp315) Antibody (人特异性) (上) 或 Caspase-9 Antibody #9502 (人特异性) (下)。

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后,细胞色素C从线粒体释放,这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化,包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处,产生p37亚单位,可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用,包括 caspase-3 and caspase-7,并启动凋亡级联反应,最终导致凋亡。

  1. Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
  2. Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
  3. Liu, X. et al. (1996) Cell 86, 147-157.
  4. Li, P. et al. (1997) Cell 91, 479-489.
  5. Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
  6. Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
  7. Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
  8. Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
  9. Sun, X. et al. (1999) J. Biol. Chem. 274, 5053-5060.
  10. MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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