Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-9 (Asp330) Antibody (Human Specific) #9501

Casp-9   casp39   caspase 9   ICE-LAP6   Mch-6  

No. Size Price
9501S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9501T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9501 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, Endogenous 37 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Cleaved Caspase-9 (Asp330) Antibody (Human Specific) detects endogenous levels of the large fragment (37 kDa with prodomain/17 kDa) of caspase-9 following cleavage at aspartic acid 330. The antibody does not recognize the uncleaved procaspase-9.

该抗体能够特异性检测内源性 Asp330 剪切的人源caspase蛋白大片段(37 kDa with prodomain/17 kDa)。该抗体与未裂解的前体 Caspase-9不发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino terminus residues surrounding to Asp330 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人源caspase-9 Asp330 周围残基相对应的肽段免疫动物后制备获得。抗体通过蛋白A和肽段亲和层析而纯化。

Western Blotting

Western Blotting

Western blot analysis of HeLa cells, untreated or staurosporine-treated (1 µM), and Jurkat cells, untreated or etoposide-treated, using Cleaved Caspase-9 (Asp330) Antibody (Human Specific) (upper) or Caspase-9 Antibody #9502 (Human Specific) (lower).

Western blot分析未处理或 staurosporine (星胞菌素) (1 µM)处理的HeLa 细胞,以及未处理或etoposide(依托泊苷)处理的Jurkat 细胞,抗体采用Cleaved Caspase-9 (Asp330) Antibody (人种特异性) (上) 或Caspase-9 Antibody #9502 (人种特异性) (下)


Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf 1. Apaf-1 mediated activation of caspase-9 involves instrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Additional cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).

Caspase-9 (ICE-LAP6, Mch6)是半胱氨酸天冬氨酸蛋白水解酶(caspase)家族的重要成员(1,2)。凋亡信号刺激后,细胞色素C从线粒体释放,这和47 kDa procaspase-9/Apaf 1有关。Apaf-1介导的caspase-9 活化,包括蛋白水解过程导致的caspase-9在Asp315处裂解并产生p35亚单位。另外一种裂解发生在Asp330处,产生p37亚单位,可以放大凋亡反应(3-6)。Cleaved caspase-9 进一步促进其他 caspase 家族成员的作用,包括 caspase-3 and caspase-7,并启动凋亡级联反应,最终导致凋亡。

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Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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