Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb #9496

casp810  

No. Size Price
9496L 300 µl ( 30 western blots ) ¥9,325.00 现货查询 购买询价
9496S 100 µl ( 10 western blots ) ¥3,780.00 现货查询 购买询价
9496T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9496 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human, 18, 41, 43 Rabbit IgG
IHC-P 1:100
F 1:100
IF-IC 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-IC=Immunofluorescence (Immunocytochemistry),

Specificity / Sensitivity

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb detects human Caspase-8 when cleaved at Asp391 (isoform A, Asp374 on isoform B). This antibody will detect cleavage products containing the pro-domain with the p18 subunit as well as the p18 subunit alone.

Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb 兔单抗能够检测人caspase-8 Asp391(亚型A,亚型B是在Asp374位)剪切的片段,该抗体可以检测包含带有P18亚单位的前体结构域和独立的P18 亚单位。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Asp391 of human Caspase-8.

该单克隆抗体是采用合成的人caspase-8蛋白Asp391周围残基相对应的多肽免疫动物生产制备。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells, untreated (blue) or etoposide-treated (green), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb compared to a nonspecific negative control antibody (red).

Western Blotting

Western Blotting

Western blot analysis of HeLa cells transfected with the death receptors Fas and DR5, or treated with cycloheximide (CHX) and TNF-alpha using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb (upper), or total Caspase-8 (1C12) Mouse mAb (lower).

IF-IC

IF-IC

Immunofluorescent staining of HeLa cells untreated or treated with cytotoxic anti-Fas Antibody, using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human Non-Hodgkin's lymphoma using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon (chronic inflammation) using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded HeLa cells, untreated (left) or Cycloheximide and TNFα-treated (right), using Cleaved Caspase-8 (Asp391) (18C8) Rabbit mAb.

Background

Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

CD95受体(Fas/APO-1)和肿瘤坏死因子受体1(TNFR1)诱导的凋亡激活了caspase-8,并导致caspase-8释放活化片段p18和p10(1-3)。活化的caspase-8 裂解并活化下游的效应caspases,如caspase-1、-3、-6和-7。Caspase-3最终引起凋亡的形态学特征变化,包括DNA的片段化和细胞固缩。

  1. Muzio, M. et al. (1996) Cell 85, 817-27.
  2. Boldin, M.P. et al. (1996) Cell 85, 803-15.
  3. Fernandes-Alnemri, T. et al. (1996) Proc Natl Acad Sci U S A 93, 7464-9.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

U.S. Patent No. 5,675,063.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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