Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Caspase-7 (Asp198) Antibody #9491

apoptosis   casp367   caspase 7   Caspase-7   caspase7   CMH-1   ICE-LAP3   Mch-3  

No. Size Price
9491S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9491T 20 µl ( 2 western blots ) ¥1,300.00 现货查询 购买询价
9491 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 20 Rabbit
IP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Cleaved Caspase-7 (Asp198) Antibody detects endogenous levels of the large fragment of caspase-7 resulting from cleavage at aspartic acid 198. The antibody does not cross-react with full length caspase-7 or with other caspases.

Cleaved Caspase-7 (Asp198) Antibody 能够检测内源性的198位天冬氨酸处裂解的caspase-7蛋白大片段。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues surrounding Asp198 in human caspase-7. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的人 caspase-7 蛋白 198位天冬氨酸处裂解的氨基末端残基相应的肽段免疫动物而生产制备,抗体通过了蛋白A和亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells and 293 cells, untreated, cytochrome c-treated (0.25 mg/ml, 1 hour) or etoposide-treated (25 µM, 5 hours), using Cleaved Caspase-7 (Asp198) Antibody.

采用Cleaved Caspase-7 (Asp198) Antibody对未处理或 cytochrome c(细胞色素c)处理(0.25 mg/ml, 1 hour) 或 etoposide (依托泊苷)处理(25 µM, 5 hours)的 Jurkat 和 293 细胞进行 western blot 分析。


Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (2-4). Caspase-7 is an effector caspase (along with caspase-2 and -3), meaning that it cleaves essential cellular machinery rather than activating other caspases (5-8). Caspase-7 is cleaved by many enzymes, including caspases-3, -6, -8, -9 and granzyme B (1,4,5). Once activated, caspase-7 cleaves many of the same substrates as caspase-3, including poly (ADP-ribose) polymerase, or PARP (2,4).

Caspase-7 (CMH-1, Mch3, ICE-LAP3)被鉴定为凋亡的主要执行者(2-4)。Caspase-7是一种效应酶(与caspase-2和caspase-3),意味着它在胞内裂解发挥必要机制,而不是激活其它caspases(5-8)。Caspase-7通过一系列酶裂解发挥作用,包括caspases-3、-6、-8、-9 和 颗粒酶B(1,4,5)。Caspase-7一旦活化,它能裂解许多与caspase-3相同的底物,包括核糖体聚合酶,或者PARP(2,4)。

  1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
  2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
  3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
  4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
  5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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