Cell Signaling Technology

Product Pathways - Apoptosis

Cleaved Drosophila ICE (drICE) (Asp230) Antibody #9478

apoptosis   drICE   Drosophila   ICE  

No. Size Price
9478S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9478 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 D. melanogaster, Endogenous 21 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Cleaved Drosophila ICE (drICE) (Asp230) Antibody recognizes endogenous levels of the large 21 kDa fragment of cleaved drICE. This antibody also detects low levels of full length drICE (35 kDa).

Cleaved Drosophila ICE (drICE) (Asp230) Antibody能够检测内源性水平的drICE剪切的21 kDa大片段。该抗体也能识别低丰度的全长drICE (35 kDa)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp230 of Drosophila ICE protein. Antibodies are purified by protein A and peptide affinity chromatography.

该多克隆抗体是采用合成的果蝇ICE蛋白近Asp230氨基末端周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from Drosophila S2 cells, untreated (-) or treated with either cycloheximide (10 μM, 5 hr; +) or actinomycin D (0.7 μM, 5 hr; +), using Cleaved Drosophila ICE (drICE) (Asp230) Antibody.

Western blot 检测果蝇S2细胞提取物,未处理 (-)或cycloheximide(放线菌酮) (10 μM, 5 hr; +) 或actinomycin(放线菌素D)(0.7 μM, 5 hr; +)处理,使用抗体为 Cleaved Drosophila ICE (drICE) (Asp230) Antibody。


Cell death in the fruit fly Drosophila melanogaster is regulated by many of the same stimuli as mammalian cell death (1). The Drosophila genome contains seven caspase genes; three encode initiator caspases and four encode effector caspases (reviewed in 2). drICE is a cysteine protease that cleaves baculovirus p35 and lamin DmO in vitro and acts downstream of rpr (3). drICE is proteolytically processed during apoptosis into active p21 and p12 subunits. Comparison of the in vivo activity between drICE and Dcp-1 has shown that drICE is a more effective inducer of apoptosis than Dcp-1, which plays a role in determining the rate of cell death (4).

黑腹果蝇的细胞死亡和哺乳动物细胞死亡一样,是受许多相同的刺激调控的(1)。果蝇的基因组包含七个caspase基因,三个编码半胱天冬酶,四个编码效应胱天蛋白酶(2)。果蝇的效应器caspase,即死亡caspase-1(DCP-1),是一个关键的细胞凋亡执行者。它参与了许多关键蛋白的蛋白水解的剪切,如多聚核酶(ADP-核糖)聚合酶(PARP)。 DCP-1的激活需要其非活动状态酶原蛋白水解成活性p22和p13片段(3)。研究比较体内drICE和DCP-1的活性表明,drICE相对于Dcp-1是一种更有效的细胞凋亡诱导剂,后者在确定细胞死亡率方面起到了重要作用(4)。

  1. Steller, H. et al. (1994) Philos Trans R Soc Lond B Biol Sci 345, 247-50.
  2. Hay, B.A. and Guo, M. (2006) Annu Rev Cell Dev Biol 22, 623-50.
  3. Fraser, A.G. and Evan, G.I. (1997) EMBO J 16, 2805-13.
  4. Florentin, A. and Arama, E. (2012) J Cell Biol 196, 513-27.

Application References

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