Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-CDK Substrate Motif [(K/H)pSP] MultiMab™ Rabbit mAb mix #9477

CDK   CDK2   CDK5   cyclin dependent kinases   general   KSP   motif   substrate  

No. Size Price
9477S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9477 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,All Species Expected, Endogenous Rabbit IgG
IP 1:50
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb recognizes phospho-serine in a KS*P motif. The antibody does not cross-react with phospho-threonine or phospho-tyrosine containing peptides/proteins.

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb兔多抗识别KS*P基序中磷酸化的丝氨酸。此抗体与包含磷酸化苏氨酸和磷酸化丝氨酸的肽段或蛋白没有交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phospho-CDK substrate peptide library. The antibody is formulated from two rabbit monoclones in order to cover a broad range of reactivity.

该单克隆抗体用合成的磷酸化CDK的底物库肽段免疫动物制备。该抗体是两个兔单克隆的混合,以便可以覆盖更大的反应范围。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS-7 cells, synchronized in G1/S, G2/M, and G0 phase of the cell cycle, using Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb.

对COS-7细胞,将其同步在G1/S、G2/M和G0期,使用Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb兔单抗进行Western blot分析其蛋白抽提物。

ELISA-Peptide

ELISA-Peptide

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb ELISA assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (S* denotes phosphorylated serine.)

Phospho-(Ser) CDKs Substrate (P-S2-100) Rabbit mAb ELISA分析:磷酸化和非磷酸化的信噪比。(S* 代表磷酸化的丝氨酸)

Background

Cyclin-dependent kinases (CDKs) are a family of Ser/Thr kinases that regulate cell-cycle transitions through their association and subsequent phosphorylation of targets in a strictly ordered fashion (1). The substrates for CDKs are proline-directed. The consensus amino acid sequence for CDK substrate is (K/R)(S*)PX(K/R), where X denotes any one of the 20 amino acids (2-4) and S* is the phosphorylation site. Phospho-(Ser) CDKs Substrate Antibody recognizes phosphorylated CDK substrates at their consensus motif, providing a powerful tool for CDK target discovery and characterization, as well as HTS drug screening for potential kinase regulators.

Cyclin-dependent kinases (CDKs)是一个丝氨酸苏氨酸激酶家族,以严格而有序的方式通过结合并磷酸化目标蛋白调控细胞周期进程(1)。CDK的底物是脯氨酸导向的。CDK底物的共有氨基酸序列为(K/R)(S*)PX(K/R),X代表任意20种氨基酸之一(2-4),S*是磷酸化位点。Phospho-(Ser) CDKs Substrate Antibody识别共有基序的磷酸化CDK底物,为CDK靶标的发现和鉴定提,及高通量(HTS)药物筛查潜在激酶调节者提供了强大的工具。

  1. Morgan, D.O. (1997) Annu Rev Cell Dev Biol 13, 261-91.
  2. Songyang, Z. et al. (1996) Mol Cell Biol 16, 6486-93.
  3. Songyang, Z. (1999) Prog Biophys Mol Biol 71, 359-72.
  4. Holmes, J.K. and Solomon, M.J. (1996) J Biol Chem 271, 25240-6.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

MultiMab is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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