Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SirT1 (D1D7) Rabbit mAb #9475

deacetylase   Sirt   Sirtuin  

No. Size Price
9475S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9475T 20 µl ( 2 western blots ) ¥1,200.00 现货查询 购买询价
9475 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 120 Rabbit IgG
IF-IC 1:400

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IF-IC=Immunofluorescence (Immunocytochemistry),

Homology

Species predicted to react based on 100% sequence homology: Chicken, Bovine, Pig, Horse,

Specificity / Sensitivity

SirT1 (D1D7) Rabbit mAb recognizes endogenous levels of total SirT1 protein. This antibody does not cross-react with other sirtuin proteins.

SirT1(D1D7 )Rabbit mAb兔单抗能够检测内源性的SirT1总蛋白水平。该抗体不会与其他sirtuin蛋白发生交叉反应。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe297 of human SirT1 protein.

该单克隆抗体是采用合成的与人源SirT1蛋白Phe297残基周围序列相对应的肽段免疫动物而生产的。

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (positive; left), WT MEF (positive; middle), and SirT1 KO MEF (right) cells using SirT1 (D1D7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.采用共聚焦免疫荧光术检测Hela细胞(阳性,左)、WT MEF细胞(阳性,中)以及SirT1 KO MEF 细胞(右) ,使用的抗体为T SirT1 (D1D7) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 鬼笔环肽进行标记(红色)。WT 和KO MEF 由Wenyi Wei, Harvard Medical School惠赠 。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb.Western blot方法检测多种细胞系的提取物,使用的抗体为SirT1 (D1D7) Rabbit mAb。

Western Blotting

Western Blotting

Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT1 (D1D7) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.Western blot方法检测SirT6 wild-type (WT) 和 knockout (KO) 小鼠胚胎成纤维细胞(MEF)的提取物,使用的抗体为SirT1 (D1D7) Rabbit mAb (上图)或 β-Actin (D6A8) Rabbit mAb #8457 (下图)。WT 和 KO MEF 由Wenyi Wei, Harvard Medical School惠赠。

Background

The Silent Information Regulator (SIR2) family of genes is a highly conserved group of genes that encode nicotinamide adenine dinucleotide (NAD)-dependent protein deacetylases, also known as class III histone deacetylases. The first discovered and best characterized of these genes is Saccharomyces cerevisiae SIR2, which is involved in silencing of mating type loci, telomere maintenance, DNA damage response, and cell aging (1). SirT1, the mammalian ortholog of Sir2, is a nuclear protein implicated in the regulation of many cellular processes, including apoptosis, cellular senescence, endocrine signaling, glucose homeostasis, aging, and longevity. Targets of SirT1 include acetylated p53 (2,3), p300 (4), Ku70 (5), forkhead (FoxO) transcription factors (5,6), PPARγ (7), and the PPARγ coactivator-1α (PGC-1α) protein (8). Deacetylation of p53 and FoxO transcription factors represses apoptosis and increases cell survival (2,3,5,6). Deacetylation of PPARγ and PGC-1α regulates the gluconeogenic/glycolytic pathways in the liver and fat mobilization in white adipocytes in response to fasting (7,8). SirT1 deacetylase activity is inhibited by nicotinamide and activated by resveratrol. In addition, SirT1 activity may be regulated by phosphorylation, as it is phosphorylated at Ser27 and Ser47 in vivo; however, the function of these phosphorylation sites has not yet been determined (9).

沉默信息调节因子(SIR2)基因家族是一类高度保守的基因,能够编码烟酰胺腺嘌呤二核苷酸(NAD)依赖性的去乙酰化酶,亦称III类组蛋白去乙酰化酶。在这些基因中,酿酒酵母Sir2是最早被发现,并且了解得最清楚的。Sir2 参与了交配型基因座的沉默,端粒维持,DNA损伤应答和细胞衰老(1)。 SirT1是一个核蛋白,是哺乳动物中Sir2的直系同源基因,与许多细胞过程调控相关联,包括细胞凋亡、细胞衰老、内分泌信号转导、糖稳态、衰老和长寿。SirT1的靶蛋白包括乙酰化的p53 (2,3)、 p300 (4)、 Ku70 (5)、 forkhead (FoxO) 转录因子(5,6)、 PPARγ (7)和PPARγ 共活化因子-1α (PGC-1α)蛋白(8)。p53和FoxO转录因子的去乙酰化会能够足额凋亡并且提高细胞存活率(2,3,5,6)。PPARγ和PGC-1α的去乙酰化能够调控肝脏中糖异生/糖酵解通路以及白色脂肪细胞中的脂肪动员来对禁食做出的应答(7,8)。SirT1去乙酰化酶活性可以被尼克酰胺抑制,而被白藜三醇活化。由烟碱禁止并且resveratrol激活deacetylase活动。另外SirT1活性可能是通过磷酸化作用来调控。因为在机内它的Ser27和Ser47 位点是磷酸化的,但是这些磷酸化位点的具体功能尚不明确(9)。

  1. Guarente, L. (1999) Nat. Genet. 23, 281-285.
  2. Vaziri, H. et al. (2001) Cell 107, 149-159.
  3. Luo, J. et al. (2001) Cell 107, 137-148.
  4. Bouras, T. et al. (2005) J. Biol. Chem. 280, 10264-10276.
  5. Brunet, A. et al. (2004) Science 303, 2011-2015.
  6. Motta, M.C. et al. (2004) Cell 116, 551-563.
  7. Picard, F. et al. (2004) Nature 429, 771-776.
  8. Rodgers, J.T. et al. (2005) Nature 434, 113-118.
  9. Beausoleil, S.A. et al. (2004) Proc. Natl. Acad. Sci. USA 101, 12130-12135.
  10. Bennett, B.T. et al. (2013) PLoS One 8, e83518.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Protocols

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0

该产品暂无评论!

我要参与评论 :

如要参与评论请先登录网站

还没有网站账户?去注册一下吧

Products

 

Applications