Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-FoxO3a (Ser318/321) Antibody #9465

AFX   forkhead   Fox03a   foxo-3a   foxo3   foxo3-a   Foxo3a  

No. Size Price
9465S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9465T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9465 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 97 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Homology

Species predicted to react based on 100% sequence homology: Chicken,

Specificity / Sensitivity

Phospho-Fox03a (Ser318/321) Antibody detects endogenous levels of Fox03a only when phosphorylated at serine 318/321. The antibody is expected to cross-react with Fox01 when phosphorylated at serine 322/325 based on the peptide sequence.

Phospho-Fox03a (Ser318/321) Antibody可以检测在Ser318/312发生磷酸化的内源性Fox03a蛋白。本抗体可与Ser322/325位点磷酸化的Fox01发生交叉反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Fox03a. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用合成的与人类Fox03a 蛋白序列相对应的肽段免疫动物生产的。抗体由protein A和肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from serum starved and serum-treated NIH/3T3 cells as well as untreated and LY294002/Wortmannin-treated MDA-MB-468 cells, using Phospho-Fox03a (Ser318/321) Antibody (upper) or Akt Antibody #9272 (lower).Western blot 分析血清饥饿的或血清处理的NIH/3T3细胞和未处理与LY294002/Wortmannin处理的MDA-MB-468细胞提取物,所用抗体为Phospho-Fox03a (Ser318/321) Antibody (上) or Akt Antibody #9272 (下) 。

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).Phosphorylation sites Ser322 and Ser325 of Fox01 are important for nuclear export and are homologous to Ser318 and Ser321 of Fox03a (9).

Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生(1-3)。在这个家族中,三个成员(FoxO1,FoxO4和FoxO3a)与线虫的同源基因DAF-16具有序列相似度,而DAF-16介导包括IGFR1,PI3K和Akt在内的信号通路(4-6)。活化的forkhead成员作为肿瘤抑制因子,促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调,而这个上调过程可以被PI3K负调控(7)。forkhead转录因子被Akt在Thr24,Ser256以及Ser319磷酸化后而失活,导致出核转运与转录因子活性的抑制(8),最终导致增殖上调。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SirT1)抑制(9)。Fox01 在Ser322和Ser325位点的磷酸化对出核转运作用非常重要,在Fox03a中这个关键位点对应的同源位点是Ser318 和 Ser321(9)。

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.
  10. Rena, G. et al. (2002) EMBO J. 21, 2263-2271.

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Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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