Cell Signaling Technology

Product Pathways - PI3K / Akt Signaling

Phospho-FoxO1 (Thr24)/FoxO3a (Thr32) Antibody #9464

AFX   FKHR   forkhead   Fox0   FOXO   FOXO 1   foxo 1a   FOXO 3   FOXO-1   FOXO-3   foxo-3a   FOXO1   foxo1 a   FOXO1a   FOXO3   foxo3-a   FOXO3a   sc-22161  

No. Size Price
9464L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9464S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9464T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9464 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 78 to 82, 95 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody detects endogenous levels of Fox01/Fox03a only when phosphorylated at threonine 24 of Fox01 or threonine 32 of Fox03a. The antibody cross-reacts with phosphorylated Fox04 at threonine 28, but not with Fox01 family members phosphorylated at other sites.

Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody可以检测在Fox01 Thr24位点和Fox03a Thr32位点发生磷酸化的内源性Fox01/Fox03a蛋白。本抗体可与Thr193位点磷酸化的Fox04发生交叉反应,但不与其它位点发生磷酸化的Fox01家族成员反应。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr28 of human Fox04. Antibodies are purified by protein A and peptide affinity chromatography.

多克隆抗体是采用与人类Fox04 蛋白Thr28 周围序列相对应的合成磷酸肽段免疫动物生产的。抗体由protein A和肽亲和层析法纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from HT29 cells, serum starved or serum treated, using Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody.

Western blot 分析血清饥饿的或血清处理的HT29细胞提取物,所用抗体为Phospho-Fox01 (Thr24)/Fox03a (Thr32) Antibody。

Background

The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9).Erk phosphorylates FoxO3a at Ser294, Ser344 and Ser425, resulting in degradation of FoxO3a through the MDM2-mediated ubiquitin-proteasome pathway. Thus, Erk promotes proliferation and tumor progression by inhibiting FoxO3a (10).。

Forkhead转录因子家族参与横纹肌肉瘤和急性白血病的发生(1-3)。在这个家族中,三个成员(FoxO1,FoxO4和FoxO3a)与线虫的同源基因DAF-16具有序列相似度,而DAF-16介导包括IGFR1,PI3K和Akt在内的信号通路(4-6)。活化的forkhead成员作为肿瘤抑制因子,促进细胞周期阻滞和凋亡。FOXO家族任何成员的表达上调都会激活细胞周期抑制剂p27 Kip1。Forkhead转录因子也参与TGF-β介导的p21 CIP1上调,而这个上调过程可以被PI3K负调控(7)。forkhead转录因子被Akt在Thr24,Ser256以及Ser319磷酸化后而失活,导致出核转运与转录因子的活性的抑制(8),最终导致增殖增加。Forkhead转录因子活性也可以被去乙酰化酶sirtuin(SIRT1)抑制(9)。ERK在 Ser294, Ser344 和Ser425位磷酸化 FoxO3后, 导致FoxO3通过MDM2介导的泛素化-蛋白酶信号通路被降解。因此Erk通过抑制FoxO3a促进了肿瘤的增殖和发展(10)。

  1. Anderson, M.J. et al. (1998) Genomics 47, 187-99.
  2. Galili, N. et al. (1993) Nat Genet 5, 230-5.
  3. Borkhardt, A. et al. (1997) Oncogene 14, 195-202.
  4. Nakae, J. et al. (1999) J Biol Chem 274, 15982-5.
  5. Rena, G. et al. (1999) J Biol Chem 274, 17179-83.
  6. Guo, S. et al. (1999) J Biol Chem 274, 17184-92.
  7. Seoane, J. et al. (2004) Cell 117, 211-23.
  8. Arden, K.C. (2004) Mol Cell 14, 416-8.
  9. Yang, Y. et al. (2005) EMBO J 24, 1021-32.
  10. Camper-Kirby, D. et al. (2001) Circ Res 88, 1020-7.

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