Cell Signaling Technology

Product Pathways - Protein Translation

Phospho-4E-BP1 (Ser65) Antibody #9451

4E-BP   4e-bp1   4EBP   4ebp1   9457   bp1   PHAS   phas-1  

No. Size Price
9451L 300 µl ( 30 western blots ) ¥8,792.00 现货查询 购买询价
9451S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9451T 20 µl ( 2 western blots ) ¥1,500.00 现货查询 购买询价
9451 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,Monkey, Endogenous 15 to 20 Rabbit
IP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-4E-BP1 (Ser65) Antibody detects endogenous levels of 4E-BP1 when phosphorylated at serine 65. This antibody has been shown to also recognize 4E-BP1 when phosphorylated at serine 101 (See application reference, Wang et al. 2003).

Phospho-4E-BP1 (Ser65) Antibody检测仅在Ser65位点磷酸化的内源性4E-BP1蛋白水平水平。该抗体也能够识别在丝氨酸101磷酸化的4E-BP1蛋白(参见应用文献, Wang et al. 2003)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of mouse 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.

通过人工合成小鼠4E-BP1 蛋白相应序列的多肽片段免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells using 4E-BP1 Antibody #9644 (lower) and Phospho-4E-BP1 (Ser65) Antibody #9451 (upper). The cells were starved for 24 hours in serum-free medium and underwent a 1 hour amino acid deprivation. Amino acids were replenished for 1 hour. Cells were then either untreated (-) or treated with 100 nM insulin (+) for 30 minutes.

使用4E-BP1 Antibody #9644 (下图)和Phospho-4E-BP1 (Ser65) Antibody #9451 (上图),免疫印迹(Western Blot)分析293细胞裂解物。细胞在无血清饥饿培养24小时,接着氨基酸缺乏培养1小时,然后再加入氨基酸培养1小时。最后细胞加入100 nM胰岛素 (+)或不加(-)处理30分钟。


Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

翻译抑制蛋白4E-BP1 (又称 PHAS-1)通过结合翻译起始因子eIF4E蛋白从而抑制帽子结构依赖的翻译。4E-BP1的过磷酸化干扰上述结合并且导致激活帽子结构依赖的翻译(1)。PI3 kinase/Akt通路和FRAP/mTOR激酶通路都调节4E-BP1蛋白的激活(2,3)。在体内4E-BP1多个位点残基被磷酸化(4)。通过FRAP/mTOR蛋白使4E-BP1在Thr37和Thr46位点磷酸化不能阻止它eIF4E结合,但这被认为是为随后在4E-BP1的Ser65和Thr70位点磷酸化进行准备(5)。

  1. Pause, A. et al. (1994) Nature 371, 762-7.
  2. Brunn, G.J. et al. (1997) Science 277, 99-101.
  3. Gingras, A.C. et al. (1998) Genes Dev 12, 502-13.
  4. Fadden, P. et al. (1997) J Biol Chem 272, 10240-7.
  5. Gingras, A.C. et al. (1999) Genes Dev 13, 1422-37.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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