Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose® Bead Conjugate) #9419

4g10   anti-phosphotyrosine. general   immobilized   immunoprecipitation   motif   phospho-tyrosine   phosphotyrosine   py20   substrate  

No. Size Price
9419S 400 µl ( 40 immunoprecipitations ) ¥2,165.00 现货查询 购买询价
9419 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
IP 1:20 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgG1

Species cross-reactivity is determined by western blot.

Applications Key: IP=Immunoprecipitation,

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate) is useful for immunoprecipitation of tyrosine-phosphorylated proteins. P-Tyr-100 is immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. The antibody does not cross-react with proteins phosphorylated on serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)在磷酸化酪氨酸蛋白免疫共沉淀分析中有用。P-Tyr-100通过碳水化合物共轭交联到琼脂糖酰肼珠而固定。此抗体与其它磷酸化丝氨酸或苏氨酸的蛋白没有交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides.



Do not use for IAP in the PhosphoScan® method. IAP immobilized antibody is specific to the PhosphoScan® Kit.

不要在PhosphoScan®方法中使用IAP。IAP固定抗体为PhosphoScan® Kit专用。



Immunoprecipitation of extracts from NIH/3T3 cells, untreated or sodium vanadate-treated (1 mM for 0.5 hour), usingPhospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate), followed by Western blot analysis using Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411.

对NIH/3T3细胞抽提液,未处理或使用1mM钒酸钠处理0.5小时,在使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411进行Western blot分析后,使用Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Sepharose Bead Conjugate)进行免疫共沉淀。


Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

酪氨酸磷酸化在细胞信号转导中扮演重要角色(1)。在肿瘤中,失调的酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成(2)。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值(3,4)。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体在高通量药物发现中为研究酪氨酸磷酸化和监测酪氨酸激酶活性提供了卓越的高灵敏的新工具。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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