Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) #9417

4g10   anti-phosphotyrosine   general   motif   phospho-tyrosine   phosphotyrosine   py   substrate  

No. Size Price
9417S 200 µl ( 40 western blots ) ¥2,165.00 现货查询 购买询价
9417 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgG1
IP 1:50
IHC-P 1:800

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin),

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) is prepared by biotinylation of the antibody via primary amines. Biotinylated antibody is useful for detection or purification of tyrosine-phosphorylated proteins using avidin-biotin binding. This antibody does not cross-react with proteins phosphorylated at serine or threonine. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated)生物素化的鼠单抗通过生物素化抗体的第一个氨基酸制备。生物素化的抗体对于通过aviddin-biotin结合的方式检测或纯化酪氨酸磷酸化的蛋白质非常有用。此抗体与其它磷酸化丝氨酸或苏氨酸的蛋白没有交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-Tyr-containing peptides . Antibody is purified by protein A chromatography.

该单克隆抗体用包含磷酸化赖氨酸的肽段免疫动物制备。该抗体使用蛋白A纯化。

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells untreated and treated with sodium vanadate (1 mM for 30 minutes), using 75 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) (A), then detected by Anti-biotin Antibody, HRP-linked #7075. Western blot analysis of the same extract using 750 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411, then detected by Anti-mouse IgG, HRP-linked Antibody #7076 (B).

对NIH/3T3细胞系抽提物,未处理或钒酸钠(1mM,30分钟)处理,使用75 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) (Biotinylated) (A)进行Western blot分析,然后用Anti-biotin Antibody, HRP-linked #7075进行检测。对同一抽提物,使用750 ng/ml Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411进行Western blot分析,然后用Anti-mouse IgG, HRP-linked Antibody #7076 (B)进行检测。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, untreated (left) or λ-phosphatase-treated (right), using Phospho-Tyrosine mAb (P-Tyr-100) (Biotinylated).

对石蜡包埋的人乳腺癌,未处理(左)或λ磷酸酶处理(右),使用Phospho-Tyrosine mAb (P-Tyr-100) (Biotinylated)进行免疫组化分析。

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

酪氨酸磷酸化在细胞信号转导中扮演重要角色(1)。在肿瘤中,失调的酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成(2)。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值(3,4)。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体在高通量药物发现中为研究酪氨酸磷酸化和监测酪氨酸激酶活性提供了卓越的高灵敏的新工具。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

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Protocols

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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