Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Tyrosine Mouse mAb (P-Tyr-102) #9416

4g10   anti-phosphotyrosine   general   motif   PBS   phospho-tyrosine   phosphotyrosine   py20   sc-7020   substrate   tyrosine   tyrosine kinase assay  

No. Size Price
9416S 200 µl ( 40 western blots ) ¥2,846.00 现货查询 购买询价
9416 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgG1
IP 1:50
F 1:400
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, F=Flow Cytometry, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-102) is a high affinity IgG1 monoclonal antibody. ELISAs using a wide variety of phospho-peptides show that P-Tyr-102 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 
 2D gel western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-102 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-102's fine specificity in terms of the sequence context in which it can recognize phospho-tyrosine seems to differ slightly from that of P-Tyr-100 #9411. P-Tyr-102 does not recognize peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Tyrosine Mouse mAb (P-Tyr-102)是一个高亲和性IgG1单克隆抗体。ELISA中对大量磷酸化肽段的实验显示P-Tyr-102结合磷酸化酪氨酸,无论周围的氨基酸序列。二维凝胶Western Blot分析过钒酸盐处理处理的细胞抽提物也显示P-Tyr-102与大量酪氨酸磷酸化的蛋白质发生相互作用。P-Tyr-102具有良好特异性,它能识别的磷酸化酪氨酸与P-Tyr-100 #9411稍有不同。P-Tyr-102不与包含磷酸化丝氨酸或磷酸化苏氨酸的肽段发生相互作用。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-Tyr-containing peptides .

该单克隆抗体用人工合成包含磷酸化赖氨酸的肽段免疫动物制备。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).

对石蜡包埋的人乳腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行免疫组化分析。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis. Proteins were separated by 2D electrophoresis prior to blotting.

对1mM过钒酸盐裂解前处理30分钟的Jurkat细胞抽提液进行Western blot分析。蛋白质在印记前在二维电泳分离。

Western Blotting

Western Blotting

Western blot analysis of extracts from sodium vanadate treated (3 mM for 0.5 hour) NIH/3T3 cells, using Phospho-Tyrosine Mouse mAb (P-Tyr-102).

对3mM钒酸钠处理0.5小时的NIH/3T3细胞抽提液使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行Western blot分析。

ELISA-Peptide

ELISA-Peptide

Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.)

Phospho-Tyrosine Mouse mAb (P-Tyr-102) ELISA分析:磷酸化和非磷酸化的信噪比。(Y* 代表磷酸化的酪氨酸)

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH/3T3 cells, untreated (blue) or pervanadate-treated (green), using Phospho-Tyrosine Mouse mAb (P-Tyr-102) compared with a nonspecific negative control antibody (red).

对NIH/3T3细胞,未处理(蓝)或过钒酸盐处理(绿),使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)和非特异性阴性对照抗体(红色),进行流式细胞仪分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-102).

对石蜡包埋的NCI-H1650移植物,未处理(左)或λ磷酸酶处理(右),使用Phospho-Tyrosine Mouse mAb (P-Tyr-102)进行免疫组化分析。

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

酪氨酸磷酸化在细胞信号转导中扮演重要角色(1)。在肿瘤中,失调的酪氨酸激酶活性可以通过产生不适当的增生和存活信号而导致恶性肿瘤和肿瘤形成(2)。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值(3,4)。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体在高通量药物发现中为研究酪氨酸磷酸化和监测酪氨酸激酶活性提供了卓越的高灵敏的新工具。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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