Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Tyrosine Mouse mAb (P-Tyr-100) #9411

4g10   anti-phosphotyrosine   general   motif   PBS   phospho antibody   phospho tyrosine   phospho-tyrosine   phosphoscan   phosphotyrosine   pY100   py20   sc-508   sc-7020   substrate   tyrosine   tyrosine kinase assay  

No. Size Price
9411S 200 µl ( 40 western blots ) ¥2,846.00 现货查询 购买询价
9411 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:2000 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgG1
IP 1:100
IHC-P 1:4800
F 1:3200
IF-F 1:1600
IF-IC 1:1600
IF-P 1:1600
E-P 1:4000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), IF-P=Immunofluorescence (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Tyrosine Mouse mAb (P-Tyr-100) is a high affinity antibody. ELISAs against a wide variety of phosphopeptides indicate that (a) P-Tyr-100 binds phospho-Tyr in a manner largely independent of the surrounding amino acid sequence. 2D gel Western blot analysis of pervanadate-treated cell extracts also shows that P-Tyr-100 interacts with a broad range of tyrosine-phosphorylated proteins. P-Tyr-100 does not cross-react with peptides containing phospho-Ser or phospho-Thr. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Tyrosine Mouse mAb (P-Tyr-100)磷酸化酪氨酸鼠单抗亲和力非常高。ELISA中对大量磷酸化肽段的实验显示P-Tyr-100结合磷酸化酪氨酸,不受周围氨基酸序列影响。二维凝胶Western Blot分析过钒酸盐(pervanadate)处理的细胞抽提物也显示P-Tyr-100与大量酪氨酸磷酸化的蛋白质发生相互作用。P-Tyr-100不与其它含磷酸化丝氨酸或磷酸化苏氨酸的肽段发生相互作用。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides .该单克隆抗体用包含磷酸化酪氨酸的肽段免疫动物制备。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).

对石蜡包埋的人乳腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。

IF-IC

IF-IC

Immunofluorescent analysis of Swiss NIH/3T3 cells, serum-starved and stimulated with lysophosphatidic acid (LPA) (10 µM for 10 minutes) and fixed with PFA, using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red) and phalloidin for F-actin (green) LPA causes heavy tyrosine phosphorylation of proteins in focal adhesions, present at the tips of actin stress fibers. (Provided by Dr. Harry Mellor, University of Bristol.)

对Swiss NIH/3T3细胞,血清饥饿和溶血磷脂酸(LPA)10 uM处理10分钟后用PFA固定,使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)(红),phalloidin用于标记F-actin(绿)LPA导致局部粘合蛋白的酪氨酸磷酸化出现在肌动蛋白纤维尖端。(Dr. Harry Mellor, University of Bristol提供)

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells treated with 1 mM pervanadate for 30 minutes prior to lysis, using P-Tyr-100 Phospho-Tyrosine Mouse mAb. Proteins were separated by 2-D electrophoresis prior to blotting.

对1mM过钒酸盐裂解前处理30分钟的Jurkat细胞抽提液使用P-Tyr-100 Phospho-Tyrosine Mouse mAb进行Western blot分析。蛋白质在印记前在二维电泳分离。

ELISA-Peptide

ELISA-Peptide

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (Y* denotes phosphorylated tyrosine.)

Phospho-Tyrosine Mouse mAb (P-Tyr-100) (HRP Conjugate) ELISA分析:磷酸化和非磷酸化的信噪比。(Y* 代表磷酸化的酪氨酸)

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of K562 cells, untreated (green) or Gleevec®- treated (blue), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) compared to a nonspecific negative control antibody (red).

对K562细胞,未处理(绿)或Gleevec®处理(蓝),使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)和非特异性阴性对照抗体(红色),进行流式细胞仪分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100).

对石蜡包埋的人肺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded NCI-H1650 xenograft untreated (left) or lambda-phosphatase-treated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100).

对石蜡包埋的NCI-H1650移植物,未处理(左)或λ磷酸酶处理(右),使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)进行免疫组化分析。

IF-IC

IF-IC

Confocal immunofluorescence analysis of HeLa cells either stimulated with 20% serum (left) or untreated (right), using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Actin filaments have been labeled with fluorescein phalloidin. Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

对HeLa细胞,20%血清刺激(左)或未处理(右),使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)(红)进行免疫组化分析。肌动蛋白用fluorescein phalloidin标记。蓝色伪彩=DRAQ5®(荧光DNA染料)。

IF-P

IF-P

Confocal immunofluorescent analysis of paraffin-embedded human lung adenocarcinoma using Phospho-Tyrosine Mouse mAb (P-Tyr-100) (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

对石蜡包埋的人肺腺癌使用Phospho-Tyrosine Mouse mAb (P-Tyr-100)(红)进行共聚焦免疫荧光分析。蓝色伪彩=DRAQ5®(荧光DNA染料)。

Background

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.

赖氨酸磷酸化在细胞信号转导中扮演重要角色(1)。在肿瘤中,上调酪氨酸激酶活性可以通过产生不适当的增生和存活信号而驱使恶变和肿瘤形成(2)。具有磷酸化酪氨酸特异性的抗体在这些研究中很有价值(3,4)。Cell Signaling Technology开发的磷酸化酪氨酸单克隆抗体在逐渐增加的酪氨酸磷酸化和高通量药物发现中监视酪氨酸激酶活性研究中是非常敏感的新工具。

  1. Schlessinger, J. (2000) Cell 103, 211-25.
  2. Blume-Jensen, P. and Hunter, T. (2001) Nature 411, 355-65.
  3. Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9.
  4. Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

DRAQ5 is a registered trademark of Biostatus Limited.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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