Cell Signaling Technology

Product Pathways - Apoptosis

c-Myc Antibody #9402


No. Size Price
9402S 100 µl ( 10 western blots ) ¥3,250.00 现货查询 购买询价 防伪查询
9402 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 57 to 70 Rabbit
IP 1:50
ChIP 1:50

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, ChIP=Chromatin IP,


Species predicted to react based on 100% sequence homology: Pig,

Specificity / Sensitivity

c-Myc Antibody detects endogenous levels of total c-Myc protein. This antibody is not recommended for detection of Myc-tagged fusion proteins (use Cell Signaling Technology cat. #2276 or #2278).

c-Myc抗体能够检测内源c-Myc总蛋白。不推荐该蛋白用于检测Myc-标签融合蛋白(推荐使用CST的#2276 或 #2278)。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues of c-Myc. Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, BaF3 and NBT-11 cells, using c-Myc Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells 48 hours following mock transfection, transfection with nonspecific (control) siRNA or transfection with c-Myc siRNA. c-Myc was detected using c-Myc Antibody #9402, and p42 was detected using p42 MAPK Antibody #9108. The c-Myc Antibody confirms silencing of c-Myc expression, and the p42 MAPK Antibody is used to control for protein loading and siRNA specificity.

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Daudi cells and either 10 μl of c-Myc Antibody or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human ATF4 promoter primers, SimpleChIP® Human NPM1 Intron 1 Primers #4779, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior including proliferation, differentiation and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3 and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes such as proliferation, transformation and prevention of apoptosis by inhibiting transcription (3,4).

Myc/Max/Mad网络成员作为转录调控因子,在细胞增殖、分化和凋亡等多种细胞行为方面发挥着重要功能(1)。这些蛋白质共享一个共同的基础螺旋-环-螺旋亮氨酸拉链(bHLH-ZIP)基序,这是二聚化和DNA结合所需的。Max最初是因其与c-Myc的互作能力而被发现,发现它对于Myc蛋白与DNA结合和激活转录过程是必须的(2)。随后,Max一直被视为转录网络的核心组成部分,与Myc和Mad家族其他成员形成同源二聚体和异源二聚体(1)。Max与Myc或Mad的互作对于转录调控和细胞行为可以有相反的作用(1)。Mad家族包括四个相关的蛋白质,Mad1、Mad2 (Mxi1)、Mad3和Mad4,以及bHLH-ZIP 家族最不相关的成员Mnt和Mga。与Myc一样,Mad蛋白严格被短半衰期调控。在一般情况下,Mad家族成员通过抑制转录干扰Myc蛋白介导的生命过程,如增殖、细胞凋亡的转化和阻止(3,4)。

  1. Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702.
  2. Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7.
  3. Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82.
  4. Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!


Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

用户评论 --- 共 0


我要参与评论 :