Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-CRMP-2 (Thr514) Antibody #9397

dihydropyrimidinase related protein 2  

No. Size Price
9397S 100 µl ( 10 western blots ) ¥3,900.00 现货查询 购买询价
9397 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 60-80 Rabbit

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting,

Specificity / Sensitivity

Phospho-CRMP-2 (Thr514) Antibody detects endogenous levels of CRMP-2 only when phosphorylated at Thr514.

Phospho-CRMP-2 (Thr514)Antibody 兔多抗识别内源性的Thr514磷酸化的CRMP-2蛋白。

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide surrounding Thr514 of human CRMP-2. Antibodies are purified by peptide affinity chromatography.

该多克隆抗体由合成的人类CRMP-2蛋白Thr514附近肽段免疫动物而制成。该抗体使用多肽亲和层析纯化而得。

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or λ phosphatase treated, using Phospho-CRMP-2 (Thr514) Antibody (upper) or CRMP-2 Antibody #9393 (lower).

Western blot分析PC12细胞(未处理,或λ磷酸酶处理)提取物,使用的抗体是Phospho-CRMP-2 (Thr514) Antibody 兔多抗(上图)或CRMP-2 Antibody 兔多抗 #9393(下图)。

Background

Collapsin Response Mediator Protein-2 (CRMP-2) is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity (1,2). CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly (3). GSK-3β inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3β (2). Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway (2). CRMP-2 also mediates semaphorin-induced growth cone collapse (4). Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3β activity in these patients (5).

Collapsin反应调节蛋白-2(CRMP-2)在神经系统的发育过程中高水平表达,在轴突生长中起着至关重要的作用,它决定轴突/树突的命运和建立神经元极性(1,2)。CRMP-2结合微管蛋白二聚体,促进微管组装,从而促进轴突伸长和分枝(3)。GSK-3β磷酸化CRMP-2的Thr514,使其失活。CRMP-2被CDK5在Ser522磷酸化,被GSK-3β在Thr518磷酸化(2)。CRMP-2的磷酸化,会降低其与微管蛋白结合的能力,可由NT-3和BDNF通过PI3激酶/ Akt的途径抑制(2)。CRMP-2也介导semaphorin诱导的生长锥崩溃(4)。CRMP-2的高磷酸化在阿尔茨海默病斑块中被发现,同时伴有升高的GSK-3β活性(5)。

  1. Gu, Y. and Ihara, Y. (2000) J Biol Chem 275, 17917-20.
  2. Yoshimura, T. et al. (2005) Cell 120, 137-49.
  3. Fukata, Y. et al. (2002) Nat Cell Biol 4, 583-91.
  4. Goshima, Y. et al. (1995) Nature 376, 509-14.
  5. Cole, A.R. et al. (2004) J Biol Chem 279, 50176-80.

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