Product Pathways - Metabolism
CAD (D2T8H) Rabbit mAb #93925
|93925S||100 µl ( 10 western blots )||￥3,100.00 现货查询||购买询价|
|93925||carrier free & custom formulation / quantity||email request|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation,
Specificity / Sensitivity
CAD (D2T8H) Rabbit mAb recognizes endogenous levels of total CAD protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CAD protein.
CAD is essential for the de novo synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3).
- Coleman, P.F. et al. (1977) J Biol Chem 252, 6379-85.
- Sigoillot, F.D. et al. (2005) J Biol Chem 280, 25611-20.
- Morin, A. et al. (2012) FASEB J 26, 460-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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