Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) #9391

general   motif   PBS   phosphothreonine   substrate   Thr-Pro   TP  

No. Size Price
9391S 100 µl ( 50 western blots ) ¥3,900.00 现货查询 购买询价
9391 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:5000 Human,Mouse,Rat,All Species Expected, Endogenous Mouse IgM
IHC-P 1:400
E-P 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) detects phospho-threonine only when followed by proline. It reacts with proteins and peptides phosphorylated at the Thr-Pro motif in an otherwise highly context-independent fashion. The antibody is phospho-specific, but does not recognize phospho-threonine in the absence of an adjacent proline. The antibody does not react with phospho-tyrosine but does react with some phospho-serine peptides containing the phospho-serine-proline motif (e.g., phospho-Elk-1). (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)识别脯氨酸之前的磷酸化苏氨酸。它与磷酸化苏氨酸-脯氨酸基序发生反应,且不与背景氨基酸序列有关。此抗体是磷酸化特异性的,但是不能识别缺乏跟随脯氨酸的磷酸化苏氨酸。此抗体与磷酸化酪氨酸没有交叉反应,但与一些包含磷酸化丝氨酸-脯氨酸模体的磷酸化丝氨酸蛋白(如磷酸化Elk-1)有交叉反应。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic phospho-threonine-proline-containing peptides . This antibody is a mouse IgM clone and can be recognized by anti-mouse Ig (whole molecule) secondary antibody.

该单克隆抗体用合成的含磷酸化苏氨酸-脯氨酸的肽段免疫动物制备。该抗体是鼠IgM克隆,可被抗鼠Ig(全分子)二抗识别。

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or nocadazole-treated (1 µg/ml for 12 hours prior to lysis), using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101). Proteins were separated by 2D electrophoresis prior to blotting.

对Jurkat细胞,未处理或1ug/ml nocadazole裂解前处理12小时,使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行Western blot分析。蛋白质在印记前在二维电泳分离。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phospho-threonine-proline motifs, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人乳腺癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析,显示包含磷酸化苏氨酸-脯氨酸模体的蛋白染色。

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untreated or serum and okadaic acid-treated, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) (left). Right panel shows total protein staining.

对COS细胞,未处理或血清和冈田酸处理,使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)(左)进行Western blot分析。右侧显示全部蛋白染色。

ELISA-Peptide

ELISA-Peptide

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) ELISAs: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* denotes phosphorylated threonine.)

Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101) ELISA分析:磷酸化和非磷酸化的信噪比。(T*代表磷酸化的苏氨酸)

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人结肠癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma control (left) or lambda phosphatase-treated (right), using Phospho-Threonine-Prolin Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人乳腺癌对照(左)和λ磷酸酶处理(右),使用Phospho-Threonine-Prolin Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma of the bladder, using Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101).

对石蜡包埋的人膀胱变形上皮细胞癌使用Phospho-Threonine-Proline Mouse mAb (P-Thr-Pro-101)进行免疫组化分析。

Background

The MAPK and CDK families of serine/threonine protein kinases play important roles in cell signaling and cell cycle control. These kinases phosphorylate threonine or serine followed by a proline residue (1-6). To study and discover new MAPK and CDK substrates, Cell Signaling Technology has developed antibodies that bind to phospho-threonine followed by proline.

MAPK和CDK丝氨酸苏氨酸蛋白激酶家族在细胞信号和周期调控中扮演重要角色。这些激酶磷酸化脯氨酸之前的苏氨酸或丝氨酸(1-6)。为了确认新的MAPK和CDK底物,Cell Signaling Technology公司开发了识别有脯氨酸之前的磷酸化苏氨酸的抗体。

As determined by ELISA using a wide variety of phospho-Thr-Pro peptides, Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101) recognizes the phospho-Thr-Pro motif in a highly context-independent fashion. It also interacts with a broad range of phospho-Thr-Pro-containing proteins as determined by western analysis of nocodazole-treated Jurkat cell extracts resolved on 2-D gels.

使用大量磷酸化苏氨酸-脯氨酸肽段进行的ELISA显示,Phospho-Threonine-Proline Monoclonal Antibody (P-Thr-Pro-101)识别磷酸化苏氨酸-脯氨酸模体,而不考虑其背景序列。对Jurkat细胞抽提液进行的二维凝胶Wester分析显示它也能与很多包含磷酸化苏氨酸-脯氨酸的蛋白质发生交叉反应。

  1. Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81.
  2. Seger, R. and Krebs, E.G. (1995) FASEB J 9, 726-35.
  3. Nurse, P. (2000) Cell 100, 71-8.
  4. Cross, T.G. et al. (2000) Exp Cell Res 256, 34-41.
  5. Yang, C.C. et al. (1998) J Protein Chem 17, 329-35.
  6. Reynolds, C.H. et al. (2000) J Neurochem 74, 1587-95.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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