Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SP1 (D4C3) Rabbit mAb #9389

sc-17824   sc-420   sc-59  

No. Size Price
9389S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
9389 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Monkey, Endogenous 90 Rabbit IgG
IP 1:50
IHC-P 1:2000
F 1:100
ChIP 1:100

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), F=Flow Cytometry, ChIP=Chromatin IP,

Specificity / Sensitivity

SP1 (D4C3) Rabbit mAb recognizes endogenous levels of total SP1 protein.

SP1 (D4C3) Rabbit mAb兔单抗能够检测内源性SP1总蛋白水平。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro593 of human SP1 protein.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using SP1 (D4C3) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

免疫组化分析人源结肠癌癌组织的石蜡切片。使用SP1 (D4C3) Rabbit mAb兔单抗,对照肽段(左图)或抗原特异性肽段 (右图)。

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SP1 (D4C3) Rabbit mAb.

使用SP1 (D4C3) Rabbit mAb兔单抗,免疫印迹(Western blot)分析不同细胞中SP1 (D4C3)蛋白水平。

Chromatin IP

Chromatin IP

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 293 cells and either 5 μl of SP1 (D4C3) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP® Human DHFR Intron 1 Primers #7531, human TIN2 intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

使用SimpleChIP®Enzymatic Chromatin IP Kit (Magnetic Beads) #9003,来自4 x 106 293细胞的交联染色质以及5 µl SP1 (D4C3) Rabbit mAb或2 µl Normal Rabbit IgG #2729进行染色质免疫沉淀实验。使用SimpleChIP® Human DHFR Intron 1 Primers #7531、human TIN2 intron 1 primers和SimpleChIP® Human α Satellite Repeat Primers #4486,浓缩的DNA通过real-time PCR定量。在每个样品中免疫沉淀DNA的数量被当做一个相对于1的总input chromatin数量的信号。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SP1 (D4C3) Rabbit mAb.

使用SP1 (D4C3) Rabbit mAb兔单抗,免疫组化分析人源肺癌组织石蜡切片。

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using SP1 (D4C3) Rabbit mAb.

使用SP1 (D4C3) Rabbit mAb兔单抗,免疫组化分析人源前列腺癌组织石蜡切片。

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using SP1 (D4C3) Rabbit mAb #9389 (blue) compared to concentration matched Rabbit (DA1E) mAb XP® Isotype Control #3900 (red). Anti-Rabbit IgG (H+L), (F(ab')2 Fragment (Alexa Fluor 488 Conjugate) #4412 was used as a secondary antibody


Specificity protein 1 (SP1) is a ubiquitously expressed transcription factor belonging to the family of C2H2-type zinc finger containing DNA-binding proteins. SP1 binds GC-rich motifs with high affinity and regulates the expression of numerous mammalian genes (1,2). It interacts with many other transcription factors, such as c-Myc, Egr1, and Stat1, and with basal transcription machinery components. SP1 interacts with chromatin-modifying factors such as histone deacetylases (HDACs) and p300 in chromatin remodeling. Transcriptional activity and stability of SP1 are regulated by post-translational modification, including phosphorylation, acetylation, ubiquitylation, and glycosylation (3). Glycosylation of SP1 following insulin treatment leads to increased nuclear localization, while glucagon treatment increases cytoplasmic SP1 levels (4-6). Investigators have found high levels of SP1 in patients with Alzheimer's disease (7).

Specificity protein 1 (SP1)是一个广泛表达的转录因子,它属于包含DNAC2H2-type锌指的DNA结合蛋白。SP1高亲和力地结合到GC富含区,并且调节哺乳动物基因的表达(1,2)。它与许多其它转录因子例如c-Myc、Egr1和Stat1以及基本的转录组件相互作用。SP1与在染色质修饰中染色质修饰因子例如组蛋白去乙酰酶(HDACs)和p300。SP1的转录活性和稳定性通过翻译后修饰被调节,包括磷酸化、乙酰化、泛素化和糖基化(3)。在胰岛素处理下SP1的糖基化导致细胞核定位的增加,而胰高血糖素处理可增加细胞质的SP1水平(4-6)。研究者发现在阿尔茨海默病(AD)中SP1是有高表达的。

  1. Kadonaga, J.T. et al. (1987) Cell 51, 1079-90.
  2. Song, J. et al. (2003) Int J Mol Med 11, 547-53.
  3. Tan, N.Y. and Khachigian, L.M. (2009) Mol Cell Biol 29, 2483-8.
  4. Majumdar, G. et al. (2003) Am J Physiol Endocrinol Metab 285, E584-91.
  5. Majumdar, G. et al. (2006) J Biol Chem 281, 3642-50.
  6. Solomon, S.S. et al. (2008) Life Sci 83, 305-12.
  7. Citron, B.A. et al. (2008) J Neurosci Res 86, 2499-504.

Application References

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