Cell Signaling Technology

Product Pathways - Neuroscience

CB1 Receptor (D5N5C) Rabbit mAb #93815

No. Size Price
93815S 100 µl ( 10 western blots ) ¥3,100.00 现货查询 购买询价
93815 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat, Endogenous 60 Rabbit IgG
IP 1:50
IF-F 1:1000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-F=Immunofluorescence (Frozen),

Specificity / Sensitivity

CB1 Receptor (D5N5C) Rabbit mAb recognizes endogenous levels of total CB1 Receptor protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of mouse CB1 Receptor protein.

IF-F

IF-F

Confocal immunofluorescent analysis of mouse hippocampus: dentate gyrus (left), CA1 region (middle), and cerebellum (right), using CB1 Receptor (D5N5C) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues using CB1 Receptor (D5N5C) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

IP

IP

Immunoprecipitation of CB1 Receptor from mouse brain extracts. Lane 1 is 10 % input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CB1 Receptor (D5N5C) Rabbit mAb. Western blot analysis was performed using CB1 Receptor (D5N5C) Rabbit mAb.

Background

The endocannabinoid system consists of the cannabinoid receptors, CB1 and CB2 receptors, the enzymes that produce and degrade the endogenous cannabinoid ligands (such as FAAH, DAG lipases, and MAG lipase), and the endocannabinoid ligands derived from the metabolism of arachidonic acid, 2-arachidonoylglycerol (2-AG) and anandamide (1-3). CB1 receptor belongs to the superfamily of G protein-coupled receptors (GPCRs) and harbors a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular tail. CB1 receptor is coupled to the Gai/o subunit of the G protein which inhibits adenylyl cyclases and regulates calcium and potassium ion channels (4). CB1 receptor is one of the most abundant GPCRs in the central nervous system. It has been show to play critical roles in the wiring of the brain during development (5), in neuronal plasticity (6), analgesia, drug abuse and metabolic homeostasis (7). In addition, CB1 receptor has been shown to interact with other GPCRs, to give rise to novel pharmacological and signaling heteromers with implication in diseases (8,9).

  1. Devane, W.A. et al. (1992) Science 258, 1946-9.
  2. Hanus, L. et al. (2001) Proc Natl Acad Sci U S A 98, 3662-5.
  3. Rozenfeld, R. (2011) Traffic 12, 12-8.
  4. Mackie, K. (2006) Annu Rev Pharmacol Toxicol 46, 101-22.
  5. Harkany, T. et al. (2008) Curr Opin Neurobiol 18, 338-45.
  6. Mackie, K. (2008) Mol Cell Endocrinol 286, S60-5.
  7. Rozenfeld, R. et al. (2011) EMBO J 30, 2350-63.
  8. Rozenfeld, R. et al. (2012) PLoS One 7, e29239.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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