Cell Signaling Technology

Product Pathways - Motif Antibodies

Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381

general   motif   PBS   phosphothreonine   substrate  

No. Size Price
9381S 100 µl ( 10 western blots ) ¥3,960.00 现货查询 购买询价 防伪查询
9381 carrier free & custom formulation / quantityemail request
Applications Dilution Species-Reactivity Sensitivity MW (kDa) Isotype
W 1:1000 Human,Mouse,Rat,All Species Expected, Endogenous Rabbit
IP 1:50
E-P 1:2000

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation, E-P=Peptide ELISA (DELFIA),

Specificity / Sensitivity

Phospho-Threonine Antibody (P-Thr-Polyclonal) detects proteins and peptides phosphorylated at threonine residues in a manner largely independent of the surrounding amino acid sequence. The antibody is phospho-specific and may cross-react with some phospho-serine-containing sequences. By ELISA, it recognizes a wide variety of threonine-phosphorylated peptides, and by 2D gel Western blot analysis, it recognizes a large number of presumably threonine-phosphorylated proteins. CST recommends the use of Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391 to detect proteins containing threonine followed by proline. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)

Phospho-Threonine Antibody (P-Thr-Polyclonal)可以识别苏氨酸残基磷酸化的蛋白质和肽段,与周围氨基酸序列磷酸化情况无关。该抗体是磷酸化特异性的,可能与其它包含磷酸化丝氨酸的序列发生交叉反应。在ELISA中,其可以识别多种苏氨酸磷酸化的肽段,在二维凝胶Western Blot中,其可以识别很多推测的苏氨酸磷酸化的蛋白。CST推荐使用Phospho-Threonine-Proline mAb (p-Thr-Pro-101) #9391来检测包含脯氨酸跟随苏氨酸的蛋白质。(美国专利号:6,441,140、6,982,318、7,259,022、7,344,714;U.S.S.N. 11,484,485;及所有国外相应专利)

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic phospho-Thr-containing peptides . Antibodies are purified by protein A and peptide affinity chromatography.


Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 45 minutes prior to lysis) and subjected to 2-D gel electrophoresis, using Phospho-Threonine Antibody (P-Thr-Polyclonal).

对未处理或0.1uM calyculin A裂解前处理45分钟的Jurkat细胞抽提液进行二维电泳,使用Phospho-Threonine Antibody (P-Thr-Polyclonal)进行Western blot分析。



Phospho-Threonine Antibody (P-Thr-Polyclonal) ELISA Assay: Signal-to-noise ratio of phospho- versus nonphospho-peptides. (T* denotes phosphorylated threonine.)

Phospho-Threonine Antibody (P-Thr-Polyclonal) ELISA分析:磷酸化和非磷酸化的信噪比。(T*代表磷酸化的苏氨酸)


Much of the dynamic behavior of cellular proteins, including the regulation of molecular interactions (1), subcellular localization (2), and transcriptional regulation (3) is controlled by a variety of post-translational modifications (4). Antibodies specific for these post-translational modifications are invaluable tools in the quest to understand normal and pathogenic molecular and cellular behavior. 
 General protein modification antibodies are designed to react with modified amino acid residues (e.g., phospho-threonine, phospho-tyrosine, acetyl-lysine, nitro-tyrosine) independently of the sequence in which they are embedded. This ability to recognize modified residues in a "context independent" fashion gives these antibodies broad reactivities, presumably conferring upon them the ability to react with hundreds of distinct proteins. This broad pattern of reactivity makes these antibodies especially valuable in multiplex analyses and target discovery programs.


Protein kinases are among the most abundant eukaryotic regulatory proteins; over 500 separate kinase genes are encoded in mammalian genomes (5,6). In spite of the importance of kinases in eukaryotic biology, relatively few of their physiological targets are known. Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381 and Phospho-Threonine (42H4) mAb #9386 provide powerful tools for discovering targets of serine/threonine kinases, for monitoring and characterizing in vitro threonine phosphorylation reactions as well as for high throughput Ser/Thr kinase drug discovery.

蛋白激酶是真核细胞调节蛋白中最多的蛋白之一;哺乳动物中有超过500个独立的激酶基因编码这些蛋白(5,6)。尽管它们在真核生物的激酶中很重要,但对它们的生理靶标还相对知之甚少。Phospho-Threonine Antibody (P-Thr-Polyclonal) #9381和Phospho-Threonine (42H4) mAb #9386为发现丝氨酸/苏氨酸激酶、监测和鉴定体外苏氨酸磷酸化反应、高通量丝氨酸/苏氨酸激酶药物发现提供了有力工具。

  1. Yaffe, M.B. and Elia, A.E. (2001) Curr Opin Cell Biol 13, 131-8.
  2. Appella, E. and Anderson, C.W. (2001) Eur J Biochem 268, 2764-72.
  3. Jenuwein, T. and Allis, C.D. (2001) Science 293, 1074-80.
  4. Krishna, R.G. and Wold, F. (1993) Adv Enzymol Relat Areas Mol Biol 67, 265-98.
  5. Venter, J.C. et al. (2001) Science 291, 1304-51.
  6. Manning, G. et al. (2002) Science 298, 1912-34.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at ptmscan@cellsignal.com.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.

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